摘要
目的:探讨姜黄素增强奥沙利铂对肠癌HT-29细胞裸鼠移植瘤的诱导凋亡作用及其机制。方法:建立耐药肠癌HT-29细胞的裸鼠皮下移植瘤模型,将荷瘤鼠随机分为Con组(生理盐水)、Oxa组(奥沙利铂7.5mg/kg)、Cur组(姜黄素50mg/kg)和Oxa+Cur组(联合用药,奥沙利铂7.5mg/kg和姜黄素50mg/kg)。腹腔注射给药,每3d1次,共8次。给药后测量肿瘤体积。免疫组织化学法检测肿瘤组织细胞增殖因子(Ki-67)、凋亡抑制因子(XIAP)和核转录因子(NF-κB)的蛋白表达。RT-PCR检测NF-κB和XIAPmRNA的表达。结果:Oxa+Cur组肿瘤体积和质量明显小于其他各组。与Con组相比较,Oxa组、Cur组和Oxa+Cur组的Ki-67蛋白表达显著下降;与Oxa组和Cur组相比较,Oxa+Cur组的Ki-67蛋白表达显著下降。与Con组相比较,Oxa组、Cur组和Oxa+Cur组的NF-κB和XIAP蛋白和mRNA的表达显著下降;与Oxa组和Cur组相比较,Oxa+Cur组的NF-κB和XIAP蛋白和mRNA的表达显著下降。结论:姜黄素可以通过下调Ki-67和NF-κB及XIAP基因表达,增强奥沙利铂对肠癌HT-29细胞移植瘤的抑制细胞增殖和诱导凋亡作用。
Objective To investigate the enhanced effect of oxaliplatin by Curcum on HT-29 cell xenograft on athymic. Methods The models of HT-29 cell xenograft on athymic mouse were established and random- ized to four groups with intraperitoneal (IP) injection of different drugs (group Con 0.9% sodium chloride), group Oxa (oxaliplatin, 7.5 mg/kg), group Cur ( Cureum 50 mg/kg)and group Oxa+Cur (oxaliplatin 7.5 mg/kg and Cur- cure 50 mg/kg in combination). The drugs were injected once every 3 days, 8 times in all. The mice were sacri- ficed 1 week after the last injection. The tumor volume and tumor weight were measured during the drug thera- py. Immunohistoehemistry (IHC) was performed to detect the expression of NF-KB, XIAP and Ki-67, RT-PCR was performed to detect the expression of NF-KB and XIAPmRNA. Results One week after the last adminis- tration, the mean tumor volume and tumor weight in group Oxa+Cur were significantly dec the other groups. IHC analysis showed the expression of NF-K B and XIAP were down reased as compared to regulated in Cur and Oxa+Cur groups as compared to the other two groups. The expression of Ki-67 was also down regulated signifi- cantly in Oxa+Cur group as compared to the other groups. RT-PCR analysis showed the expression of NF-KB and XIAP mRNA in Con and Oxa+ Cur groups were down regulated significantly as compared to the other groups. Conclusion Curcum can enhance the anti-tumor effect of oxaliplatin on colorectal cancer xenograft. Downregulation of NF-KB and XIAP is possibly one of the mechanisms.
出处
《中国中西医结合外科杂志》
CAS
2013年第2期149-152,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine