强精胶囊对精索静脉曲张大鼠附睾功能性指标及精子质量的影响

Effects of Qiangjing Capsule on Epididymal Functional Indicators and Their Sperm Quality in Varicocele Rats

目的:观察强精胶囊对精索静脉曲张大鼠附睾功能性指标及精子质量的影响。方法:100只青春期雄性SD大鼠,随机抽取10只为假手术组,90只缩窄左肾静脉建立精索静脉曲张模型,随机分为模型组、强精胶囊高中低剂量组、五子衍宗胶囊组和少腹逐瘀胶囊组。分别给予相应药物灌胃,观察大鼠附睾精子密度与活力的变化,检测附睾α-糖苷酶的含量(葡萄糖氧化酶法)和L-肉毒碱的含量(DTNB法)。结果:模型组大鼠附睾精子活力与密度低下、α-糖苷酶与L-肉毒碱含量低下;强精胶囊高剂量组精子活力与密度、α-糖苷酶与L-肉毒碱含量较中、低剂量组、五子衍宗胶囊组、少腹逐瘀胶囊组明显增高(P<0.01)。结论:强精胶囊可以改善模型大鼠精子密度与活力,提高附睾α-糖苷酶与L-肉毒碱的含量。

Abstract： Objective To observe the effects of Qiangjing Capsule on the epididymal functional indicators and their sperm quality in the varicocele rats. Methods One hundred adolescent male SD rats were random- ly assigned to two groups, Sham-operated group（10 rats）and Varicocele（VC） model group（90 rats）established by narrowing the left renal vein. After four weeks, successful VC was confirmed in 72 rats which were randomly di- vided into the following groups： model group（MG）, Qiangjing capsule high（QHG）, medium（QMG） and low dose groups（QLG）, Wuzi Yanzong capsule group（WYG）, and Shaofu Zhuyu capsule group（SZG）. In each group, rats got intragastric administration respectively with saline, Qiangjing capsule high, medium and low dose, Wuzi Yan- zong capsule, and Shaofu Zhuyu capsule（10 mL/kg once a day for four weeks）. The rats epididymal sperm densi- ty and dynamic changes were observed. The rats epididymal ct-glucosidase content by using glucose oxidase method and L-carnitine content by using DTNB method was detected. Results Epididymal sperm vitality and density of MG rats were low, c^-glucosidase and L-carnitine contents of model group were in low level. Sperm motility and density, ct-glucosidase and L-carnitine contents of QHG were significantly higher than those of QMG, QLG, WYG,and SZG（P 〈 0.01）. Conclusion The Qiangjing capsule can improve the model rats＇sperm density and vitality, improve the epididymal ct-glucosidase and L-carnitine content levels.