胃癌组织中15-PGDH与COX-2的表达特征及其临床意义

Expression and clinical significance of 15-hydroxyprostaglandin dehydrogenase and cyclooxygenase-2 in gastric cancer

目的探讨15-羟基前列腺素脱氢酶(15-PGDH)和环氧合酶-2(COX-2)在胃癌中的表达及两者的相关性,并探讨与临床病理特征的关系,评价15-PGDH及COX-2在胃癌发生、发展中的作用及其意义。方法随机收集80例胃癌手术患者的癌组织及相应癌旁正常组织,应用免疫组织化学方法检测15-PGDH及COX-2的表达特征并进行评分,进一步分析两者的相关性及与胃癌临床病理参数的关系。结果胃癌组织中15-PGDH的表达水平显著降低甚至缺失而COX-2的表达水平显著升高,结合胃癌的临床病理学特征统计分析表明,15-PGDH的低表达与COX-2的高表达均与胃癌的分化程度、TNM分期以及有无淋巴结转移密切相关,且15-PGDH与COX-2的表达呈显著负相关。结论胃癌中15-PGDH和COX-2的共同作用可能是胃癌发生、发展及浸润转移的重要机制之一,同时检测15-PGDH和COX-2的表达水平将有助于判断胃癌的恶性程度及其预后。

Objective To investigate the expression and clinical significance of 15-hydroxyprostaglandin dehydrogen- ase （15-PGDH） and cyclooxygenase-2 （COX-2） in gastric cancer, study the correlation of both the expression of 15- PGDH and COX-2, and evaluate the role of 15-PGDH and COX-2 in gastric cancer occurrence and development. Meth- ods Immunohistochemistry was used to detect the expression of 15-PGDH and COX-2 proteins in 80 gastric cancer tis- sues and adjacent non-tumor tissues. Further statistic analysis were explored to value their relation with the clinicopatho- logical parameters and the correlation between themselves. Results 15-PGDH expression was decreased or even absen- ted while COX-2 was over-expressed in gastric cancer tissues compared with the corresponding non-tumor tissues. Fur- ther analysis about their relationship with clinicopathological parameters revealed that the low expression of 15-PGDH was significantly related with the differentiation grade, TNM stage and lymph node metastasis of gastrc cancer. However, COX-2 expression just had the opposite pattern. Spearman analysis showed that significant negative correlation exsited between COX-2 and 15-PGDH immunoreactivity with r = - 0. 539 （P 〈 0.01 ）. Conclusion 15-PGDH and COX-2 play important roles in gastric cancer. Determination of the two molecules＇ expression in gastric cancer tissues can be helpful to judge the degree of malignancy, metastasis phenotype and prognosis of gastric cancer.