巨噬细胞移动抑制因子在不同分级的乙型肝炎肝硬化患者外周血中的检测及临床意义

Test and clinical significance of MIF in peripheral serum of liver cirrhosis with hepatitis B of different classification

目的检测巨噬细胞移动抑制因子(MIF)在Child-Pugh分级的乙型肝炎肝硬化患者外周血中的含量,并探讨其临床意义。方法选择102例乙型肝炎肝硬化患者作为实验组,并按Child-Pugh分级分成A、B、C三级,选择24例健康体检者作为对照组,采用酶联免疫吸附(ELISA)法测定两组血清中MIF的含量。结果实验组MIF含量明显高于对照组,且随着Child-Pugh分级的递增,MIF的含量逐渐增加,差异有统计学意义(P<0.05)。结论乙型肝炎肝硬化患者血清中MIF的含量与Child-Pugh分级的程度密切相关,MIF能够反映患者肝细胞功能及预后,具有重要的临床意义。

Objective To test the content of macrophage migration inhibitory factor （MIF） in peripheral serum of liver cirrhosis with hepatitis B of Child-Pugh classification and discuss its clinical significance. Methods 102 patients of liver cirrhosis with hepatitis B as the experiment group were divided into A, B, C three grades as Child-Pugh classifi- cation. 24 cases of normal subjects were as the control group. MIF was detected by double antibody sandwich enzyme- linked immunosorbent assay （ELISA） method in peripheral serum of two groups. Results The content of MIF in exper- iment group was higher than that in the control group, and the MIF was high gradually along with the increase of Child- Pugh classification, there was a statistic significance （P 〈 0.05）. Conclusion The content of MIF is correlated with the Child-Pugh classification of liver cirrhosis with hepatitis B. MIF can reflect indirectly the function of liver cell and patient＇ s prognosis, and it has an important clinical significance.