巢蛋白在成年大鼠室周器官细胞的表达

Nestin protein expresssion in the cells of circumventricular organs in the adult rat

目的：观察巢蛋白在成年大鼠室周器官细胞的表达，并根据巢蛋白阳性表达细胞的位置、大小及形态特点进行分类。方法：成年SD雄性大鼠，经心灌注后，用免疫组织化学检测巢蛋白在室周器官细胞的表达。结果：在正中隆起（64．32％±8．55％）、穹隆下器（15．76％±2．16％）、连合下器（40．15％±6．16％）和最后区（28．85％±4．53％）都可见大量的巢蛋白阳性表达细胞。根据细胞所在位置、大小和形态将其分为5类。Ⅰ类是室管膜细胞，部分室管膜细胞有长10-180μm的胞突；Ⅱ类细胞较小，细胞及细胞核呈圆形或卯圆形，有1-2个纤细长达20-30μm的胞突；Ⅲ类细胞胞体较Ⅱ类大，细胞及细胞核不规则，胞质浅染，无胞突；Ⅳ类细胞及细胞核呈圆形或卵圆形，大部分细胞无胞突，部分细胞有长4-15μm的胞突；Ⅴ类细胞胞体较大，细胞及细胞核呈圆形或卯圆形，有1-3个粗长的突起，并可见2-3级分支，胞突长50-100μm。结论：成年大鼠室周器官有大量巢蛋白阳性表达细胞，有的是具有增殖和分化潜能的神经干细胞／祖细胞，有的是巢蛋白阳性表达的神经元。

Objective： To identify nestin protein expresssions in the cellg of circumventricular organs （CVOs） in the adult rats and its classification according to their location, size and morphological characteristics. Methods： In the adult male SD rats, after conventional heart perfusion, CVOs were detected by immunohistochemical staining for nestin positive ceils. Results： The large numbers of nestin positive expressing cells were found in median eminence （ME） （64. 32%±8. 5%）, subfornical organ （SFO） （15.76%±2.16%）, subcommissural organ （SCO） （40. 15%±6.16%） and area postrema（AP） （28.85%± 4.53%）. According to their location, size and morphological characteristics, the cells were classified to five classes. Class I was ependyma cells, some ependyma cells had long 10-180 μm processes. Class Ⅱ was the ceils with a very small cell soma and few sparse long 20-30 μm processes. Class Ⅲ was the cells bigger than Class Ⅱ cells with irregular nuclei and without processes. Class Ⅳ was the cells with spherical nuclei and without processes, hut a few cells had long 4-15 μm processes. Class V was the ceils with a very large cell soma and one to four long 90-100μm offshoot processes. Conclusion： Abundent nestin positive expressing ceils were found in CVOs of adult rats. One species may he neural stem/progenitor cells, which can proliferate and differentiate to new neurons and/or glia. Another specieg may he nestin-expressing neurons.