摘要
目的:探讨肿瘤坏死因子相关的凋亡诱导配体(TRAIL)及核转录因子-κB(NF-κB)在大鼠肝癌发生过程中的表达及意义。方法:应用免疫组织化学SP法,对二乙基亚硝胺(DEN)诱发的大鼠肝癌发生过程中TRAIL及NF-κB的动态表达进行检测。结果:二乙基亚硝胺诱发的肝癌为肝细胞癌,大鼠肝癌癌变过程大致经过肝细胞损伤期、肝细胞增生-硬化期和肝细胞癌变期3个阶段。在正常大鼠肝、损伤期及增生硬化期偶见少量肝细胞呈TRAIL阳性表达,进入癌变期,TRAIL阳性细胞数量增多,主要位于癌结节内。在正常大鼠肝,偶见少量肝细胞呈NF-κB阳性表达,随着肝癌发生发展,阳性表达细胞逐渐增多,至诱癌晚期,可见大量NF-κB阳性表达细胞,均比正常肝组织表达高。结论:TRAIL及NF-κB可能与肝癌的发生发展密切相关。
Objective.. To investigate the expression and role of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) and nuclear factor-κB (NF-κB) during hepatocarcinogenesis in rats. Methods: The dynamic expression of TRAIL and NF-~B during hepatocarcinogenesis induced by diethytnitrosamine (DEN) in rats was measured by using immunohisto- chemical SP method. Results: Hepatocellular carcinoma was induced by DEN. The procession of hepatocarcinogenesis in this model included three-stage hepatic toxin lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis. Only a few TRAIL positive cells were shown ocassionally in the normal liver tissues, toxin lesion and hepatic proliferation/cirrhosis stages. On the hepatic carcinogenesis stage, more TRAIL positive cells were seen mainly located within cancer nodules. An increasing tendency of NF-κB expression was shown from the normal liver to precancerous to cancerous tissues with a progress of rat hepatocarcinogenesis and a large number of NF-κB positive cells was shown at the terminal cancer stage compared to the lower expression in the normal liver tissues, Conelusion: The results suggest that TRAIL and NF-κB in hepatocytes are closely related to bepatocarcinogenesis,
出处
《解剖学杂志》
CAS
CSCD
北大核心
2013年第2期163-165,共3页
Chinese Journal of Anatomy
基金
国家自然科学基金(81073123)
山东省自然科学基金(ZR2009CL003,Q2008C01)