大鼠肝癌发生过程中肿瘤坏死因子相关的凋亡诱导配体及核转录因子-κB的表达

Expression of tumor necrosis factor-related apoptosis inducing ligand and nuclear factor-κB during hepatocarcinogenesis in rats

目的：探讨肿瘤坏死因子相关的凋亡诱导配体（TRAIL）及核转录因子-κB（NF-κB）在大鼠肝癌发生过程中的表达及意义。方法：应用免疫组织化学SP法，对二乙基亚硝胺（DEN）诱发的大鼠肝癌发生过程中TRAIL及NF-κB的动态表达进行检测。结果：二乙基亚硝胺诱发的肝癌为肝细胞癌，大鼠肝癌癌变过程大致经过肝细胞损伤期、肝细胞增生-硬化期和肝细胞癌变期3个阶段。在正常大鼠肝、损伤期及增生硬化期偶见少量肝细胞呈TRAIL阳性表达，进入癌变期，TRAIL阳性细胞数量增多，主要位于癌结节内。在正常大鼠肝，偶见少量肝细胞呈NF-κB阳性表达，随着肝癌发生发展，阳性表达细胞逐渐增多，至诱癌晚期，可见大量NF-κB阳性表达细胞，均比正常肝组织表达高。结论：TRAIL及NF-κB可能与肝癌的发生发展密切相关。

Objective.. To investigate the expression and role of tumor necrosis factor-related apoptosis inducing ligand （TRAIL） and nuclear factor-κB （NF-κB） during hepatocarcinogenesis in rats. Methods： The dynamic expression of TRAIL and NF-~B during hepatocarcinogenesis induced by diethytnitrosamine （DEN） in rats was measured by using immunohisto- chemical SP method. Results： Hepatocellular carcinoma was induced by DEN. The procession of hepatocarcinogenesis in this model included three-stage hepatic toxin lesion, hepatic proliferation/cirrhosis and hepatic carcinogenesis. Only a few TRAIL positive cells were shown ocassionally in the normal liver tissues, toxin lesion and hepatic proliferation/cirrhosis stages. On the hepatic carcinogenesis stage, more TRAIL positive cells were seen mainly located within cancer nodules. An increasing tendency of NF-κB expression was shown from the normal liver to precancerous to cancerous tissues with a progress of rat hepatocarcinogenesis and a large number of NF-κB positive cells was shown at the terminal cancer stage compared to the lower expression in the normal liver tissues, Conelusion： The results suggest that TRAIL and NF-κB in hepatocytes are closely related to bepatocarcinogenesis,