模拟糖尿病大鼠红细胞粘附的研究

RESEARCH ON THE ADHESION OF ERYTHROCYTES IN STZ-INDUCED DIABETIC RATS

目的 :观察糖尿病时红细胞的粘附 ,探讨红细胞粘附对糖尿病微血管病变的影响。方法 :STZ诱发大鼠糖尿病 ,FITC体外标记糖尿病红细胞后输给正常大鼠 ,荧光显微镜观察标记的糖尿病红细胞在正常大鼠软脑膜微血管中的流动 ;透射电镜观察糖尿病大鼠脑皮质微血管内红细胞的超微结构。结果 :与正常红细胞相比 ,FITC标记的糖尿病红细胞在血流正常的大鼠脑微血管中可以较长时间停留在内皮细胞表面 ,不被血流冲走 ,提示红细胞对微血管内皮细胞有粘附 ;电镜显示 ,糖尿病大鼠红细胞常常塞满毛细血管腔 ,红细胞膜与内皮细胞膜紧密相贴 ,其间缺乏应有的血浆层间隙 ,并可见高电子密度的结节状结构。可能是红细胞的“粘附结节”。结论 :糖尿病大鼠红细胞对脑微血管内皮细胞有粘附并首次观察到糖尿病时在脑微血管中红细胞的“粘附结节”。

Aim: To investigate the adhesion of erythrocyte to endothelial cells and their influence on the development of microangiopathy in diabetes. Methods: Diabetes in rats were induced by STZ. Erythrocytes were isolated and labeled with FITC, then transfused to normal rats. The movement of FITC labeled-RBC within microvessels were observed on pial mater under intravital fluorescent microscope. The ultrastructure of erythrocytes in brain of diabetic rats were observed by transmission electron microscope. Results: FITC-labeled diabetic erythrocytes were found stopping at capillary for longer time, and sticking to the surface of endothelial cells at local microvessels even though the blood stream were rapid. Ultrastructural study of erythrocytes in cerebral microvessels of diabetic rats showed that the tiny thin plasma layer between erythrocytes and endothelial cell was absent, and nodular structure with high electron density were revealed. Conclusion: It showed that the adhesion of diabetic erythrocytes to endothelial cells both in vivo and in ultrastructural level,“adhesion nodule”was revealed at the first time in diabetic erythrocytes within rat cerebral microvessels.