红豆杉细胞时相与其次生代谢强度的关系

Relationships between the strength of secondary metabolite of Taxus cell and cell phase in suspension culture system

研究了处于不同时相的红豆杉细胞经诱导后生活力、生物量、紫杉醇含量及几个与次生代谢有关的生理生化指标的差异 ,对细胞时相与次生代谢强度的关系进行了探讨。结果表明 ,在细胞培养的第7d(延迟期 )进行诱导 ,细胞的活力、POD活性、H2 O2 、生物量、紫杉醇单位含量均高于第 14d(对数期 )进行诱导 ,显著高于第 2 1d(稳定期 )开始诱导。说明在第 7d(延迟期 )进行诱导时 ,细胞对诱导子的反应灵敏度较高 ,次生代谢启动的强度更大 ,在第 7d(延迟期 )

The changes of the life ability,biomass,taxol content,POD activity and H 2O 2 content of Taxus cells were studied when thdy were induced at different cell phase.The results indicated that when the cells in lag phase were induced,the life ability,biomass,taxol content,POD activity and H 2O 2 content increased more than that of cells which were induced in exponential phase and markedly higher that of cells which were induced in stationary phase.