红酵母RY-98产类胡萝卜素培养基的优选及其发酵生理学研究

Studies on the selection of carotenoids fermentation medium for RY-98 strain of Rhodotorula and its fermentative physiology

对红酵母RY - 98菌株产生类胡萝卜素的培养基组成和培养条件进行了初步研究 ,并对发酵过程作了动态分析。结果表明 ,培养基组成、糖浓度和添加剂以及培养基初始pH值、摇瓶装量与培养时间等均对该菌细胞生物量和类胡萝卜素产量有影响 ,其中ZS添加剂的加入可以明显促进菌体类胡萝卜素的形成 ,这是研究新发现。在初步优化的培养基组成 (葡萄糖 4 0g/L、玉米浆 15g/L、(NH4 ) 2 SO4 2g/L、ZS添加剂 1g/L)和培养条件下 ,菌体生物量与类胡萝卜素含量分别为 2 7mg/mL、4 98 2 μg/g ,发酵液产色素能力为 13 3mg/L。通过观测发酵过程生理现象的变化 ,认为该菌类胡萝卜素是菌体的次生代谢产物 ,主要形成于对数生长期的末期和稳定期。

The fermentation medium of carotenoids from RY-98 strain of Rhodotorula was selected.The compositions of optimal medium were as follows:glucose 40g/L,corn steep liquor(CSL)15g/L,(NH 4) 2SO 42g/L,additive ZS 1g/L,and initial pH6.0.The carotenoids of RY-98 strain showed a distinct increase if add ZS in the medium.The optimal medium volume in 250mL flask was 60mL.27mg dry cell/mL medium and 498.2μg carotenoids/g dry cell were produced by strain RY-98 with above-mentioned conditions after growing at 28℃ for 60h.The growth,pH,the synthesis of carotenoids and carbohydrate source using were also examined.