小诺霉素单组份菌种选育及发酵条件研究

BREEDING OF SINGLE COMPONENT SAGAMICIN-PRODUCING STRAIN AND STUDIES ON FERMENTATION CONDITIONS

以棘孢小单孢菌Ａ－２３为出发菌株，用常规诱变育种为主，结合综合处理方法，如原生质体，紫外线照射，电融合再生，激光照射，在８－甲氧基补骨酯素存在下用近紫外光照射，氯化锂和紫外线复合处理等方法，以及自然分离纯化，经多代选育，得到高产菌株ＭＳ—１１６，其发酵后小诺霉素主组分含量为８５％以上；同时研究了最佳发酵条件和培养基配方，进行了３０ｔ发酵罐放大试验。

Micromonospora echinospora A-23 was screened through protoplast UV-ray, electrofasion regeneration, near ultraviolet light in presence of 8-methoxypsoralen, combined tratment of LiCl and UV-ray, etc, and isolated single colonies with natural selection. A high and stable sagamictu-producing strain MS-116 was obtained through several-decade generations of the treatment. The main component content increased to over 85%. The culture medium and the effective factors on fermentation was also studied. Trial-production by 30 ton-fermentor indicated that the strain is high and stable is high and stable sapamicin-producing strain for industrial production.