烟草花粉母细胞减数分裂前期Ⅰ肌动蛋白的免疫细胞化学定位(简报)

STUDIES ON THE DISTRIBUTION OF ACTIN DURING MEIOTIC PROPHASE I OF NICOTIAN A RUSTICA L.

肌动蛋白是一种主要的收缩蛋白,在肌肉细胞中是广泛存在的,而在非肌肉细胞中,如在藻类细胞以及高等植物的根尖细胞、表皮细胞中,也存有肌动蛋白。在花粉萌发过程中,肌动蛋白丝集聚成束,对于花粉管的形成和受精过程有着重要作用。但已有的工作,多数限于动物细胞和植物体细胞中,关于高等植物的生殖细胞中的报道则比较少见。

The Pollen Mother Cells of Nicotiana Rusti-ca L. were studied using the techniques of im-munofluorescence microscopy and protein A-col-loidal gold IEM. Immunofluorescence microscopy indicates the presence of actin in both nuclei and cytoplasm. IEM observation shows that gold particles are present in cytoplasm, chromatin and cytomictic channels. These results indicate that actin has some relation with chromatin condensing at synizesis. Moreover, actin may also play an important role in cytomixis.