水培与土培离子液体[C_2mim][Val]对小麦的影响

Effects of ILs [C_2 mim] [Val] on Wheat Under Two Culture Conditions

为考察离子液体在水和土壤不同环境介质中对高等植物的潜在毒性,分别采用水培和土培试验方法研究了不同浓度(0、100、200、300、400、500 mg/kg)的离子液体1-甲基-3-乙基-咪唑缬氨酸盐([C2mim][Val])对小麦发芽率(7 d)及生理特性(8～18 d)的影响。实验结果表明:两种培养方式下,离子液体在浓度≥200mg/kg时对小麦种子萌发产生显著的抑制作用,且抑制效应具有典型的剂量依赖型特点。水培胁迫13 d,离子液体浓度≥300 mg/kg处理,及土培胁迫18 d,离子液体浓度≥400 mg/kg时,均造成小麦幼苗叶片SOD、POD酶活性降低,MDA含量显著升高。表明较高浓度的离子液体对高等植物具有一定的毒害作用,水培比土培加重了离子液体对小麦的毒害程度。

This paper showed the potential toxic effect of ILs in different environmental media （ water and soil） on higher plants, by using water cuhure and soil cuhure respectively. The effee, t of ILs [ C2mim ] [ Val] on wheat germination rate （ 7 d） and physiologieal characteristics（8-18d） was studied in different concentrations （0, 100, 200, 300, 400, and 500 mg·kg^-1）. The results show that the wheat seedlings were inhibited obviously in the concentration of ,nore than 200 mg·kg^-1 of ILs. In addition, the inhibited effeel was dose-dependent under both euhivations. After treated 13 d with water at the concentration more than 300 mg·kg^-1 ILs and 18 d with soil at more than 400 mg·kg^-1 ILs, the activities of SOD and POD of the wheat seeding leaves both decreased while MDA increased obviously, which proves that the higher concentration of ILs has a certain toxic effect ou higher plants and the toxic effect of ILs on wheat under water culture was greater than that of soil culture.