多色流式细胞术对骨髓增生异常综合征患者外周血免疫表型的分析

Analysis of Immunophenotype of Peripheral Blood by Multi-color Flow Cytometry in Myelodysplastic Syndrome

目的了解骨髓增生异常综合征(MDS)患者外周血细胞免疫表型特征。方法采用多色流式细胞术CD45/SSC双参数散点图设门方法,对38例MDS组患者及24例Non-MDS组患者外周血原始细胞群、粒细胞群及单核细胞群免疫表型进行分析。结果 MDS组患者外周血原始细胞群、粒细胞群及单核细胞群均存在异常免疫表型。MDS组和Non-MDS组外周血原始细胞群中CD34+细胞聚集的患者为16例和3例,比例分别占42.1%和12.5%,差异有统计学意义(P<0.05)。两组中,外周血原始细胞群表达CD11b+细胞的患者为10例(26.3%)和1例(2.6%)。MDS组高于Non-MDS组,差异有统计学意义(P<0.05)。MDS组粒细胞群中侧向散射光(SSC)的平均荧光强度和CD10的几何平均荧光强度(G-MFI)分别为7.4±2.8,4.0±3.4;Non-MDS组分别为9.5±2.7,8.2±6.6,MDS组均明显低于Non-MDS组,差异有统计学意义(P<0.01)。结论 MDS患者外周血细胞上存在异常的免疫表型,其特点有助于MDS与Non-MDS疾病的鉴别。

Objective To investigate the characteristics of immunophenotype in patients with myelodysplastic syndrome(MDS) using the samples of peripheral blood(PB).Methods The antigen expression of myeloblasts,granulocytes and monocytes in samples of peripheral blood from 38 patients with MDS and 24 patients with Non-MDS were detected by multicolor flow cytometry with four color and CD45/SSC gating.Results The abnormal immunophenotypes were existed in myeloblasts,granulocytes and monocytes in samples of PB from MDS group.The results were showed that 16 patients(42.1%) have clustered distribution of CD34+ cells on PB myeloblasts in MDS group and 3 patients(12.5%) in Non-MDS group(P<0.05).The abnormal expression of CD11b+ cells on PB myeloblasts in MDS group(26.3%) was higher than that of in Non-MDS group(2.6%)(P<0.05).The numbers of SSC and the Geometric mean fluorescent intensity(G-MFI) for CD10 on granulocytes in MDS group(7.4±2.8,4.0±3.4) were significantly lower than that of in Non-MDS(9.5±2.7,8.2±6.6)(P<0.01) respectively.Conclusioren The abnormal Immunophenotypes can be found in patients with MDS using PB.It may be helpful to distinguish MDS from Non-MDS.