冠状动脉内放射抑制球囊扩张术后ERK1/2活性及c-fos基因表达

Inhibition of ERK1/2 activity and c-fos mRNA after coronary artery balloon injury by intracoronary radiation in swine

本文旨在观察血管内放射对冠状动脉球囊扩张术后细胞外信号调节激酶 1/ 2 (ERK1/ 2 )及c fos基因表达的影响。实验对猪的左冠状动脉前降支或回旋支行球囊行过度扩张术 ,术后即刻通过血管内放射治疗系统对猪冠状动脉损伤局部给予 2 0Gy的放射剂量 ,分别在术后 3d和 30d处死动物 ,留取目标血管组织。通过反转录 聚合酶链反应定量检测血管内放射对球囊扩张术后血管组织c fosmRNA的表达 ,采用生化方法测定血管组织ERK1/2的活性。结果表明血管内放射治疗明显抑制了球囊扩张术后 3dERK1/ 2活性和c fosmRNA的表达 (2 0 5 % ,P <0 0 1;47 7% ,P <0 0 5 ) ,而对球囊扩张术后 30d的ERK1/ 2活性和c fosmRNA表达无显著影响。这提示ERK1/ 2和c fos可能参与了血管内放射抑制球囊扩张术后早期再狭窄过程。

The effect of intracoronary radiation on extracellular signal regulated kinase 1/2 (ERK1/2) activity and c fos mRNA after coronary artery balloon injury was investigated in swine. Twenty three swines were randomly divided into a radiation group and a control group after coronary balloon over stretch. The dilated segments in the radiation group were exposed to a dose of 20 Gy by a catheter based radiation system. The animals were sacrificed at 3 (6 swines from each group) and 30 days (6 swines from radiation group and 5 from control group) after the operation. The injured segments were processed to examine c fos gene expression by reverse transcription polymerase chain reaction (RT PCR) and to measure the activity of ERK1/2 biochemiscally. Intracoronary radiation decreased significantly the ERK1/2 activity and gene expression of c fos in the radiation treated animals 3 days after coronary balloon injury (20 5%, P <0 01; 47 7%, P <0 05), but neither ERK1/2 activity nor c fos gene expression was significantly affected by endovascular radiation in animals 30 days after balloon injury. Therefor, both ERK1/2 and c fos may be involved in inhibiting restenosis.