摘要
应用BAC文库，采用基于分子标记的染色体着陆（marker-based chromosome landing）和染色体步查（chromosome walking）等手段，建立了包含有水稻抗稻瘟病基因Pi-2（t）的物理图谱，该物理图谱由22个BAC克隆组成，遗传跨度8cM，而物理距离为925kb，该物理图谱的构建不仅为进一步分离和克隆该基因打下了基础，同时也可为分子标记辅助选择育种选择抗稻瘟病新材料提供新的标记。
In an effort to clone a rice blast resistance gene, Pi*2(t), a BAC contig consisting of 22 BAC clones covering the whole Pi-2(t) region, was constructed usingmarker-based chromosome landing and chromosome walking. The genetic span of this physical map is 8cM, but the physical size is 925kb. The physical map forms the base of further isolation and cloning of Pi-2(t) gene, it will provide new markers for molecular marker-aided selection of new blast resistance materials in rice.
基金
国家自然科学基金!(批准号:39780016)
