类风湿关节炎相关自身抗体谱检测比对

Quality of Rheumatoid Arthritis Associated Antibodies Tests in China

目的了解类风湿关节炎相关自身抗体谱在我国的检测水平及各种自身抗体检测的准确率。方法由北京协和医院风湿免疫科向参与比对的实验室寄发比对品,各实验室在规定时间内将实验方法及检测结果回报。比对项目包括抗环瓜氨酸肽抗体、类风湿因子、抗角蛋白抗体、抗核周因子抗体。比对品发放和结果分析采用随机双盲法,由北京协和医院对各家医院检验结果进行分析并将结果反馈给各实验室。结果抗环瓜氨酸肽抗体、类风湿因子、抗角蛋白抗体、抗核周因子抗体正确率的平均值分别为98.6%、95.3%、73.4%及79.2%。结论目前全国大部分实验室开展了类风湿关节炎相关自身抗体谱的检测,其中抗环瓜氨酸肽抗体和类风湿因子的检测正确率高,而抗角蛋白抗体、抗核周因子抗体的检测水平有待提高。

Objective To investigate the quality of rheumatoid arthritis associated antibodies tests in China. Methods Laboratory quality control materials were sent to the hospitals participated in this study by the Department of Rheumatology of Peking Union Medical College Hospital （PUMCH）. The test methods, results and reagents used in all laboratories were reported to PUMCH in pre-specified time. The tests included anti-cyclic citrullinated peptide （anti-CCP） antibody, rheumatoid factor （RF）, anti-keratin antibodies （AKA） and antiperinuclear factor （APF）. The control samples were distributedand the testing results were analyzed by randomly and double-blindly. Results The correct rate of anti-CCP antibody, RF, AKA and APF were 98. 6%, 95.3%, 73.4%, and 79. 2%, respectively. Conclusions At present, most of the laboratories are carrying out the tests for auto-antibodies of rheumatoid arthritis. The correct rate for examing anti-CCP antibody and RF is high, while the correct rate of APF and AKA needs to be improved.