血塞通对局灶性脑梗死大鼠海马Nogo-A表达的影响

Effects of Xuesaitong Lyophilized Powder on Expression of Nogo-A in Hippocampus in Rats with Focal Cerebral Ischemia

目的探讨血塞通对大脑中动脉阻塞(MCAO)模型SD大鼠脑组织的保护、促进功能恢复的机理,以及血塞通不同剂量的疗效。方法使用改良的Longa方法建立大鼠大脑中动脉阻塞(MCAO)模型,随机分组,选取不同恢复时间点,观察不同组别大鼠的神经功能、海马的病理形态学以及Nogo-A蛋白表达的变化。结果与正常组比较,模型组术后Nogo-A表达变化均有增高,并随时间出现下降趋势,血塞通组不同时间点的神经功能损伤、病理形态改变、Nogo-A基因的表达水平均低于同期模型组。结论血塞通可以有效降低No-go-A基因表达,促进中枢神经功能的恢复;同时发现血塞通大剂量组作用优于小剂量组。

Objective： To observe the expression of Nogo-A in rat hippocampus at different times in SD rat model of middle cerebral artery occlusion and to explore the protective mechanism after cerebral ischemia and dose-effect relationship of Xuesaitong Lyophilized Powder.Methods： The SD rats were divided randomly into different groups after the model of middle cerebral artery occlusion（MCAO） was established by improved Longa EZ method.In these models,some indices were observed including the change of nerve functions,pathomorphology and the expression of Nogo-A in rat hippocampus at different time points.Results： Compared with the control group,the expression of Nogo-A was increased in the model group which presented the descending trends with times.These indicators in the Xuesaitong Lyophilized Powder group were lower than those in the model group at different times such as the nerve function damage,the pathomorphology and the expression of Nogo-A.Conclusion： Xuesaitong Lyophilized Powder can reduce the expression of Nogo-A effectively and promote reconstruction of central nervous system.The effect of the large dose of Xuesaitong Lyophilized Powder group is better than that of small dose of Xuesaitong Lyophilized Powder group.