响应面法优化油茶粕中茶皂素提取工艺

Response Surface Optimization Extraction Process of Tea Saponin from Tea Seed Cake(Camellia oleifera)

采用单因素实验结合响应面法,优化了从油茶粕中提取茶皂素的工艺参数。单因素实验最佳条件:乙醇体积分数为70%、提取时间为3 h、提取温度为70℃、料液比为1∶4。在单因素的基础上,选取乙醇体积分数、提取时间、料液比为影响因子,以茶皂素提取得率作为响应值,进行3因素3水平响应面分析。结果表明:茶皂素最佳提取条件是乙醇体积分数为72%、提取时间为3.8 h、提取温度为70℃、料液比为1∶4.5,提取得率预测值为14.54%,验证实测值为14.22%,与预测值相对误差为2.20%。

Single-factor and response surface analysis methodology were used to optimize the extraction process parameters of tea saponin from tea seed cake （ Camellia oleifera）. The optimum conditions of the single factor experiment were following ethanol volume fraction of 70%, extraction time of 4h, extraction temperature of 70℃ and solvent-to-feed ratio of 4：1, respectively. Based on single-factor experiments, three independent variables, ethanol volume fraction, extraction time and solvent-to-feed ratio, were selected as affecting factors during extraction. Response surface analysis method was applied to determine the effect of central composite experiment design. The optimum extraction conditions were found to be extracted with solvent-to-feed ratio 1 ：4.5 at 70℃ using 72% ethanol for 3.8 h. The predicted extraction yield of tea saponin was 14.54% ,while the actual extraction yield of tea saponin was 14.22% ,with relative error of 2.20%.