超声提取水杉叶总黄酮的工艺研究

Research on the Ultrasonic Extracting Technology of Total Flavonoids from the Leaves of Metasequoia

目的:研究水杉中总黄酮的超声提取工艺,并优化其提取工艺。方法:以总黄酮含量为考察指标,采用正交试验法,考察加醇量、提取次数、提取时间、乙醇浓度4个因素对提取工艺的影响。结果:最佳提取工艺为A3B1C1D3,即药材加乙醇20倍量,提取1次,提取时间15min,乙醇浓度85%。结论:超声提取法是一种理想的提取水杉总黄酮的方法。

Objectives： The research aimed to study the ultrasonic extraction technology of total flavonoids from the Leaves of Metasequoia and optimize its extraction process. Methods： With dry extract rate and flavonoid content as the inspection indicators, orthogonal test was used to investigate the influence of four extracting factors （ethanol volume, extracting , extraction time and ethanol consistence） on the extracting process. Results： The optimum extracting process was A3B1CID3, i.e. The ethanol volume being 20 times of the weight of medicinal material, extraction for one times and 15 minutes each time, and 85% ethanol consistence. Conclusion： Ultrasonic extraction method was a ideal extraction method of total flavonoids from the Leaves of Metasequoia.