紫外分光光度法测定金边瑞香枝和叶中总黄酮的含量

Content of Total Flavonoids in the Branch and Leaves of Daphne Odora Var.Marginata by UV Spectromertry

目的:建立金边瑞香枝和叶中总黄酮含量的测定方法,为该品的质量控制提供依据。方法:以葛根素为对照品,采用紫外分光光度法在254nm波长处测定样品的吸光度。结果:对照品在0～100mg/L范围内呈良好线性关系,回归方程为Y=0.0040X+0.0052,R=0.9997。金边瑞香枝和叶中总黄酮含量分别为0.1262%和0.2615%。结论:该方法操作稳定,结果准确、可靠,重现性好,适合金边瑞香枝和叶中总黄酮含量的测定。

Objective ： To establish a method for the determination of Content of Total flavonoids s in the Branchs and Leaves of Daphne odora Var. Marginata and To provide reference for control the quality. Methods： Puerarin was selected as the reference material, used the UV spectromertry methods to determine content of total flavonoids in the s Branchs and leaves with a detection wavelength at 254nm. Results ： The calibration curve was a good linear at the range of 0 - 100mg/L, and the curve was Y = 0. 0040X ＋ 0. 0052, R = O. 9997. The content of Branchs was about 0. 1262% and content of leaves was about 0. 2615%. Conclusion ： This method is stable, the result is accurate, reliable, and has good repeatability, and The UV spectromertry is suitable for the determination of total fla- vonoids in the Branehs and leaves of Daphne edora Var. Marginata.