松针提取物对高脂血症大鼠载脂蛋白的影响

Effects of pine needle extract on hyperlipidemia rat apolipoprotein

目的探讨松针提取物对实验性高脂血症大鼠的预防作用及机制。方法清洁级近交系40日龄Wistar雄性大鼠40只,根据体重随机分成4组,每组10只:正常对照组饲喂基础饲料;高脂饲料对照组饲喂高脂饲料;松针提取物低、高剂量干预组饲喂高脂饲料,并分别给予50、150mg/kgBW松针提取物灌胃,另两组大鼠给予同体积生理盐水灌胃,连续用药10周。分别于实验前及实验的第5、10周末禁食12h后眶静脉取血,测定血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白B(ApoB)、载脂蛋白A-I(ApoA-I),末次取血后将实验大鼠处死,取主动脉及肝脏组织作形态学检查。结果与高脂对照组比较,高剂量松针提取物干预组TC、TG、LDL-C、ApoB、ApoB/ApoA-I显著降低(P<0.01),HDL-C、ApoA-I显著升高(P<0.05)。与高脂对照组比较,松针提取物组干预组大鼠肝脂肪空泡明显缩小,动脉斑块缩小,两干预组相比,高剂量松针提取物干预组效果更明显(P<0.05)。结论松针提取物具有降低ApoB、ApoB/ApoA-I,阻止动脉硬化早期主动脉粥样硬化发展的作用。原花青素是发挥此作用的主要物质。

Objective To investigate the effect of pine needle extract on preventive effect and mechanism in experimental hyperlipidemia rats. Methods Forty clean grade inbred male 40- day-old male Wistar rats, according to body weight were randomly divided into four groups（N： 10） ： The normal group was fed with normal diet; The high fat diet control group was fed a high fat diet; Pine needle extract low- and high-dose intervention group was fed a high fat diet, and were given 50,150mg/kg BW pine needle extract orally, the other two groups of rats were given the same volume of saline, 10 weeks of continuous medication. The orbital vein blood was col- lected after 12h fasting before the experiment and the experiment 5,10 weekend to determine ser- um total cholesterol, triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, apolipoprotein B, apolipoprotein A-I. After the last blood experimental rats were killed, and the aorta and liver tissue were for morphological examination. Results Pine needle extract compared with the high-fat control group, high-dose intervention groups TC, TO, LDL- C of ApoB, ApoB/ApoA-I decreased significantly （P〈 0.01）, HDL-C, ApoA-I significantly in- creased （P〈0.05）. Compared with the high-fat control group, pine needle extract intervention group rat liver fat vacuoles reduced significantly and arterial plaque was narrowing. The effect of high doses pine needle extract intervention group was more significant（P〈0.05）. Conclusion Pine needle extract can reduce ApoB, ApoB/ApoA-I and prevent the development of aortic atherosclerosis in the early stages of atherosclerosis. Procyanidins is a main substance to play this role.