摘要
目的:探讨滋补肝肾方药复健片对局灶性脑梗死大鼠健侧大脑皮质RhoA、ROCK-2基因和蛋白表达的影响。方法:Wistar大鼠随机分为假手术组、模型组和药物组,各组根据观测时间点再分为术后4周末、术后5周末两个亚组。采用电凝法闭塞模型大鼠右侧大脑中动脉,采用RT-PCR方法观测RhoA mRNA、ROCK-2 mRNA表达,采用Western-blot观测RhoA、ROCK-2蛋白表达。结果:在各观测时间点,三组左侧大脑运动皮层组织中RhoA基因和蛋白表达均无显著性差异;而模型组ROCK-2的表达则在术后4周末低于假手术组(蛋白表达,P<0.05;基因表达,P<0.01),至术后5周末,与假手术组已无显著性差异;药物组ROCK-2的表达则在术后4周末和5周末均低于其余两组(与假手术组相比,P<0.01;与模型组相比,除在术后4周末蛋白表达,P<0.01,其余均为P<0.05)。结论:局灶性脑梗死后,未损伤侧大脑皮质的ROCK-2基因和蛋白表达均下调,复健片可抑制相应大脑皮质的ROCK-2表达,这可能是复健片促进未损伤侧大脑重塑的重要机制之一;而RhoA的表达虽然未见明显变化,但不排除活性发生变化。
Objective:To investigate the effect of Fujian tablet tonifying the liver and kidney on the expression of RhoA and ROCK-2 in the contralesional cortex of model rats with focal cerebral ischemia. Methods :Wistar rats were randomly divided in- to three groups:sham surgery group,model group and administration group. Each group were randomly divided into two sub- groups according to observation time including the end of 4 weeks and the end of 5 weeks after operation. The right middle cerebral artery of model rat was occluded by electric coagulation. Gene and protein expression of RhoA and ROCK-2 were de- tected respectively by RT-PCR and Western-blot methods. Results :There were no differences in the expression of gene or pro- tein of RhoA in the contralesional cortex between these three groups. The expression of ROCK-2 in model group was weaker than sham operation group only at the end of 4 weeks after operation(P〈0.05 in protein expression and P〈0.01 in gene expression) and the expression of ROCK-2 in administration group was weaker than other two groups at the end of both 4 weeks and 5 weeks (vs sham group,P〈0.01, vs model group P〈0.05,except at the end of 4 weeks in protein expression,P〈0.01). Conclusion:The expression of ROCK-2 reduced in contralesional cortex of rats with focal cerebral ischemia and Fujian tablet can inhibit the expression of ROCK-2,whieh maybe an important mechanism of Fujian tablet to promote eontralesional cortex plasticity. But it is not proved whether activity of RhoA in eontralesional cortex was changed by cerebral isehemia.
出处
《山东中医药大学学报》
2013年第2期145-147,共3页
Journal of Shandong University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:81273701)