云南兔儿风小极性成分GC-MS分析

GC-MS Analysis of Low Polar Components from Ainsliaea yunnanensis

目的:分析云南兔儿风乙醇提取物中的小极性成分。方法:云南兔儿风干燥茎叶用乙醇超声提取,得到的浸膏水溶后依次用石油醚、乙酸乙酯和正丁醇进行萃取;石油醚萃取部分进行正相硅胶柱层析,得到石油醚∶丙酮=50∶1～20∶1的洗脱流分即为小极性成分。采用气相色谱-质谱(GC-MS)联用技术对小极性成分进行分离鉴定,并运用气相色谱峰面积百分比法确定其相对百分含量。结果:从云南兔儿风小极性部位共鉴定了50个化合物。结论:该结果为全面认识云南兔儿风的化学成分和药理作用提供了理论依据。

Objective：To analyze the chemical constituents of low polar components from ethanol extract of Ainsliaea yunnanensis.Methods：The air-dried stems and leaves of Ainsliaea yunnanensis were extracted with ethanol using ultrasonic extration.After removal of solvent under reduced pressure,the residue was suspended in H2O,and then partitioned sequentially with petroleum ether,EtOAc and n-BuOH,respectively.Then the petroleum ether fraction was chromatographed on normal phase silica gels eluting with a gradient increasing Me2CO in petroleum ether.Then the components of low polarity were separated from the petroleum ether-Me2CO（50∶ 1~20∶ 1）fraction and identified by GC-MS,and the relative contents of the components were determined with area percentage method.Results：50 constituents were identified from the low polar components of Ainsliaea yunnanensis.Conclusion：The research provides a theoretical basis for the study of chemical constituents and pharmaceutical activities of Ainsliaea yunnanensis.