两种纯化工艺对白芍水提液化学成分的影响规律研究

Study on the Rule of Influence of Two Purification Methods on the Chemical Compositions in Aqueous Solution of Paeoniae Radix Alba

目的:探索壳聚糖絮凝澄清工艺与醇沉工艺对白芍水提液化学成分的影响规律。方法:采用HPLC建立白芍水提物的指纹图谱,以指纹图谱中各峰对应组分的表观含量与相对表观含量为评价指标比较2种纯化工艺对白芍水提液化学成分含量的影响。结果:壳聚糖絮凝澄清工艺对水提液中大极性成分的保留优于醇沉工艺,对中等极性成分的保留与醇沉工艺相近,而对小极性成分的保留则差于醇沉工艺。结论:该实验结果可为合理选用上述2种纯化工艺用于中药水提液的纯化提供一定的指导。

Objective：To explore the rule of influence of chitosan flocculation clarification and alcohol precipitation on the chemical compositions in aqueous extract of Paeoniae Radix Alba.Methods：The fingerprints of aqueous extracts of Radix Paeoniae Alba were established by HPLC method,and the influences of the two purification methods on the chemical compositions in aqueous extract of Paeoniae Radix Alba were compared with the apparent content and relative apparent content of the composition as evaluation indexes.Results： The chitosan flocculation clarification was superior to alcohol precipitation for keeping the polar compositions in aqueous extract,close to alcohol precipitation for keeping the medium polar compositions,and inferior to alcohol precipitation for keeping the lower polar compositions.Conclusion：The experiment result provides evidence for reasonably selecting above two purification methods to purify aqueous extract of Chinese medicinal herbs.