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Blastocyst Formation and Chromosome Statuses of Reconstructed Embryos Derived from Interspecies Somatic Cell Nuclear Transfer (iSCNT)

Blastocyst Formation and Chromosome Statuses of Reconstructed Embryos Derived from Interspecies Somatic Cell Nuclear Transfer (iSCNT)
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摘要 Objective To analyze the blastocyst formation and chromosome statuses of reconstructed embryos derived from human-goat interspecies somatic cell nuclear transfer （iSCNT）, exploring the development retardant factors. Methods Human specific point probes cep2, cep6, tel2 and 13q14.2, 21q22.13 combining fluorescence in-situ hybridization （FISH） technology were used to test trophectoderm cells of blastocyst and blastomeres of development arrest nuclear transfer （NT） embryos. Results A total of 209 reconstructed embryos were recovered, and the rate of blastocyst formation was 3.8% （8/209）. FISH signals showed that chromosomal abnormalities were present in 2 blastocysts （2/8） and 146 development arrest embryos （146/201）. Conclusion The rate of blastocyst formation is low, and reconstituted embryos of development arrest showed extensive chromosome abnormalities, suggesting that a chromosomal mechanism may underlie their developmental arrest. Objective To analyze the blastocyst formation and chromosome statuses of reconstructed embryos derived from human-goat interspecies somatic cell nuclear transfer （iSCNT）, exploring the development retardant factors. Methods Human specific point probes cep2, cep6, tel2 and 13q14.2, 21q22.13 combining fluorescence in-situ hybridization （FISH） technology were used to test trophectoderm cells of blastocyst and blastomeres of development arrest nuclear transfer （NT） embryos. Results A total of 209 reconstructed embryos were recovered, and the rate of blastocyst formation was 3.8% （8/209）. FISH signals showed that chromosomal abnormalities were present in 2 blastocysts （2/8） and 146 development arrest embryos （146/201）. Conclusion The rate of blastocyst formation is low, and reconstituted embryos of development arrest showed extensive chromosome abnormalities, suggesting that a chromosomal mechanism may underlie their developmental arrest.
作者 Xiao-hong GUAN Yun FENG Xiao-wei LU Jian-quan CHEN Yi-juan SUN Hong-ying SHA
机构地区 Reproductive Medical Center Shanghai Transgenic Research Center Department of Neurosurgery National Key Laboratory for Medical Neurobiology
出处 《Journal of Reproduction and Contraception》 CAS 2013年第1期38-44,共7页 生殖与避孕（英文版）
基金 supported by Grants from Special Fund for Excellent Young University teachers in Shanghai 2012 Shanghai Science and Technology Developmental Foundations (Grant number: 09ZR1419000)
关键词 interspecies somatic cell nuclear transfer （iSCNT） preimplantation embryonicdevelopment fluorescence in-situ hybridization （FISH） chromosomal status interspecies somatic cell nuclear transfer （iSCNT） preimplantation embryonicdevelopment fluorescence in-situ hybridization （FISH） chromosomal status
分类号 R321 [医药卫生—人体解剖和组织胚胎学]
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  • 1Fragouli E, Wells D, Thornhill A, et al. Comparative genomic hybridization analysis of human oocytes and polar bodies. Hum Reprod, 2006, 21(9):2319-28.
  • 2Mantzouratou A, Mania A, Fragouli E, et al. Variable aneuploidy mechanisms in embryos from couples with poor reproductive histories undergoing preimplantation genetic screening. Hum Reprod, 2007, 22(7): 1844-53.
  • 3Gardner DK, Lane M, Stevens J, et al. Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril, 2000, 73(6): 1155-8.
  • 4Menasha J, Levy B, Hirschhorn K, et al. Incidence and spectrum of chromosome abnormalities in spontaneous abortions: new insights from a 12-year study. Genet Med, 2005, 7(4):251-63.
  • 5Fragouli E, Lenzi M, Ross R, et al. Comprehensive molecular cytogenetic analysis of the human blastocyst stage. Hum Reprod, 2008, 23(11):2596-608.
  • 6Alfarawati S, Fragouli E, Coils P, et al. The relationship between blastocyst morphology, chromosomal abnormality, and embryo gender. Fertil Steril, 2011, 95(2):520-4.
  • 7Alikani M, Calderon G, Tomkin G, et al. Cleavage anomalies in early human embryos and survival after prolonged culture in-vitro. Hum Reprod, 2000, 15(12):2634-43.
  • 8Munne S. Chromosome abnormalities and their relationship to morphology and development of human embryos. Reprod Biomed Online, 2006, 12(2):234-53.
  • 9Snijders RJ, Holzgreve W, Cuckle H, et al. Maternal agespecific risks for trisomies at 9-14 weeks' gestation. Prenat Diagn, 1994, 14(7):543-52.
  • 10Iwasaki S, Hamano S, Kuwayama M, et al. Developmental changes in the incidence of chromosome anomalies of bovine embryos fertilized in vitro. J Exp Zool, 1992, 261(1):79-85.

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Journal of Reproduction and Contraception
2013年 第1期

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