细菌16SrDNA基因PCR检测在败血症诊断的临床研究

Clinical study on application of 16SrDNA PCR in diagnosis of septicemia

目的建立细菌16SrDNA基因半巢式PCR检测方法,并与血培养方法比较,评估其在临床败血症的诊断价值。方法针对16SrDNA高度保守区设计半巢式PCR引物,并对92例临床疑为败血症的患者血标本同时进行血培养和16SrDNA基因检测。结果 92例疑为败血症患者16SrDNA基因检测阳性45例,阳性率为48.9%;血培养检测阳性标本24例,阳性率为26.1%。经χ2检验,PCR检测的灵敏性明显高于血培养(P<0.05)。结论半巢式PCR方法检测细菌16SrDNA基因具有高敏感、高特异及快速的优点,是一种易于推广的早期败血病诊断方法。

Objective To establish the semi-nested PCR for detection of bacteria 16SrDNA genes and to assess its clinical application in the diagnostic of septicemia compared with the methods of blood culture. Methods Specific semi-nested PCR primers were designed according to the conserved sequence of 16SrDNA, and a total of 92 patients with suspected sepsis were carried on blood 16SrDNA PCR as well as blood culture. Results Among the 92 patients examined, 45 （48.9%） were positive for the 16SrDNA gene, while 24 （ 26. 1% ） were positive for blood culture. The sensitivity of PCR was significantly higher than blood culture by X2 test. Conclusions The methods of semi-nested PCR have the advantages of high sensitivity, high specificity and rapid, which is easy to be popularized in the early diagnosis of septicemia.