摘要
RNA干扰(RNAi)是利用双链RNA特异性地降解同源靶标mRNA,从而特异性地阻断相应基因表达的现象,它属于转录后水平的基因沉默(PTGS)机制。RNAi广泛存在于生物体中,是2l世纪的研究热点之一。近年来,RNAi在植物功能基因组学研究中发挥着越来越重要的作用。在此对RNAi的作用机制和特点,及其在植物基因功能分析、作物品种改良、抗病虫害和抗逆性等方面的研究进行概述,旨在为RNAi在植物研究上的应用提供参考。
RNA interference （ RNAi ） belongs to the post-transcriptional gene silencing phenomenon in which double-stranded RNA triggers the degradation of homologous mRNA, thereby specific to suppression the corresponding gene expression. RNAi is a phenomenon that commonly existed in organisms, and it has become one of the hotspots of scientific research in 21＂ century. In recent years, RNAi plays a more and more important role in plant functional genomics. We reviewed characteristics and action mechanism of RNAi, as well as the research progress in aspects including the plant gene function analysis, the crop variety improvement and the resistance to disease or pests, in order to provide reference for RNAi application in plant research.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第3期48-52,共5页
Biotechnology Bulletin
关键词
RNA干扰
双链RNA
基因沉默
植物
应用
RNA interference
Double-stranded RNA
Gene silencing
Plant
Application
