UV-B辐射对小麦叶片蛋白的影响

UV-B Radiation Affects Protein Expression of the Wheat’s Leaf

为研究增强的UV-B辐射对植物的影响,选取了生长于中国北方的经济作物冬小麦为研究对象,采用双向电泳的方法,分析了经UV-B辐射后小麦叶片蛋白的变化。结果显示,经UV-B辐射后,第4天、第8天的小麦叶片蛋白变化明显,双向电泳图谱显示发现15个蛋白差异点;通过质谱鉴定了3个蛋白差异点,分别为铜/锌过氧化物歧化酶、钙调素、Rubiso大亚基结合蛋白α亚基。结果表明增强的UV-B辐射可以通过调节小麦叶片基因编码蛋白而调节植物生长。

The reduction in average global ozone layer may cause the enhance of solar ultradiation adiation （ UV-B ） that reach to the ground surface. We selected the cash crops of winter wheat which grown in northern China to studied the influence of UV-B radiation on plants. Using the method of two-dimensional electrophoresis to analysis changes of the protein in wheat leaves by UV-B radiation. The results showed that wheat leaf protein of the first four days, eight days changes significantly. The two dimensional gel electrophoresis revealed 15 differentially expressed polypeptides, 3 of which were successful identified by mass spectrometry, including Copper/Zinc Superoxide dismutase, Calmodulin, Rubisco large subunit binding protein subunit alpha. The results showed that enhanced UV-B radiation can be regulating the gene encoding protein of wheat leaf to adjust plant growth.