7个大穗型小麦-黑麦代换系间杂交后代的形态学与SSR分析

Analysis of Seven Big Spike Types from the Hybrids of Wheat-Rye Substitution Lines by Morphology and SSR

对从小麦-黑麦代换系5R/5A与1R/1D杂交后代中选育的大穗型品系1-5、1-6、1-7、1-8、1-9、1-10、1-11进行形态学与SSR分析。结果表明,7个品系田间表现遗传性稳定,且具有黑麦大穗、抗病等优良性状;利用黑麦特异引物PSC119.1确定7个品系均导入了黑麦染色体片段,引物SCM138扩增结果表明,在1-6、1-7、1-8、1-9、1-10中导入了黑麦染色体5RS片段,引物SCM120、TSM604可以在7个品系中稳定扩增出黑麦5R长臂和1R短臂特异片段。以上结果可为小麦遗传育种与品质改良提供基础材料。

We analyzed the big spike lines 1-5, 1-6, 1-7, 1-8, 1-9, 1-10 and 1-11 by morphology and SSR, which were bred from hybrids of wheat-rye substitution lines 5R/5A and 1R/1D. The results showed that the genetic performance of seven strains were stability, and had the good rye traits, such as big spike, resistance to disease. Seven lines contained genetic element of rye chromosome that were determined by rye-specific primers PSC119.1. The amplification results of primer SCM138 showed that 1-6, 1-7, 1-8, 1-9 and 1-10 contained chromatin of 5RS. The primer SCMI20 and TSM604 could amplify rye specific fragment stably in seven lines that proved the lines contained the rye ehromatin of 5RL and 1RS. These results provide the material foundation for wheat genetic breeding and quality improvement.