能源植物续随子肌动蛋白Actin基因全长序列的克隆及序列分析

Cloning and Sequence Analysis of Actin Gene from Energy Plant Euphorbia lathyris

利用同源克隆和RACE技术克隆得到能源植物续随子肌动蛋白Actin基因的cDNA全长序列,该基因cDNA全长1 743 bp(命名为ElActin,GenBank登录号为KC182753)。序列分析表明,ElActin开放阅读框长1 134 bp,编码377个氨基酸,5'非编码区247 bp,3'非编码区362 bp。ElActin与其他植物Actin基因的核苷酸序列同源性达到82%以上,氨基酸序列的同源性达97%以上。

Actins exist widely in plants. In this study, homologous cloning and RACE technology were employed to obtain the Actin full-length cDNA sequence of Euphorbia lathyris （ named ElActin, GenBank accession No. KC182753 ） . Sequence analysis showed that E1Actin contained a 1134 bp open reading frame （ ORF ） encoding a 377 amino acid residues, a 5＇-UTR of 247 bp and a 3＇-UTR of 362 bp. Compared with other plants, the homology of nacleotide sequence and amino acid sequence is above 82% and 97%, respectively.