硝酸银对根癌农杆菌介导的木薯遗传转化的影响

Effect of Silver Nitrate on Agrobacterium Mediated Genetic Transformation of Cassava

旨在提高木薯遗传转化效率,以成熟培养10-15 d的华南木薯SC8品种的胚状体子叶为外植体,研究硝酸银在根癌农杆菌介导的木薯遗传转化中的影响。结果表明,硝酸银对木薯SC8胚状体子叶愈伤形成有明显的抑制作用,对不定芽的分化有显著的促进作用。硝酸银的浓度在2-8 mg/L,对未经农杆菌处理的SC8胚状体子叶不定芽的分化率从58.5%提高到87.5%;愈伤组织形成率从92%下降到15.3%。对经农杆菌处理的SC8胚状体子叶不定芽的分化率从55.3%提高到85.3%;愈伤组织形成率从87.5%下降到8.5%。同时发现,经农杆菌转化后不定芽诱导所需硝酸银的最适浓度高于未经农杆菌处理的不定芽诱导所需硝酸银最适浓度,前者为8 mg/L,后者为6 mg/L。硝酸银对木薯SC8的遗传转化有促进作用。

It was to improve the conversion efficiency of cassava genetic transformation. The effect of silver nitrate on the Agrobacterium mediated genetic transformation of cassava Was assessed by the maturation of 10-15 days of Southern China cassava SC8 embryoid cotyledon as expiants. The resuks showed that silver nitrate could improve the regeneration frequency and reduce callus formation in scg. The use of silver nitrate at concentrations between 2 and 8 mg/L increased shoot organogenesis frequency from 58.5% to 87.5% while callus formation decreased from 92% to 15.3% without the Agrobacterium cocultivation ; and shoot organogenesis frequency increased from 55.3% to 85.3% while callus formation decreased from 87.5% to 8.5% with Agrobacterium cocultivation. Meantime, the silver nitrate optimum concentration of adventitious buds induction by the Agrobacterium mediated genetic transformation was higher than that without the Agrobacterium treatment, the former was 8 mg/L, the latter was 6 mg/L. The application of silver nitrate can stimulate cassava SC8 genetic transformation.