杉木炭疽病拮抗菌HY32的筛选及其应用

Screening and Application of the Antagonistic Bacterium HY32 Against Colletotrichum gloeosporioides

采用组织分离法从杉木炭疽病叶中分离到一株病原菌,命名为CSUFTCC F0101。经形态观察、致病性测定和分子生物学鉴定确证为杉木胶孢炭疽菌(Colletotrichum gloeosporioides)。采集杉木健康枝条,通过平板对峙法初筛、发酵液法复筛,获得1株具有较强拮抗活性的细菌,编号为HY32,其抑菌带宽度达到10 mm以上,发酵液的抑菌活性为81.7%。抗菌谱测定表明,该拮抗细菌对油茶炭疽病菌、油茶软腐病菌、油茶根腐病菌和油茶叶枯病菌等4种病原真菌也具有较强的抗菌活性。对拮抗菌HY32的防病效果进行室内盆栽试验,结果表明,该拮抗菌对杉木炭疽病具有显著防治效果(≥60.0%),与对照组具有显著的差异。

We isolated pathogenic bacteria by means of tissue isolation. Through morphology, pathogenicity determination and phylogenetic analysis, it showed that the cause of fir anthrax pathogen is Colletotrichum gloeosporioides. Acquisition of Sugiki Kenkosugimie, through the flat confrontation method screening, fermentation liquid compound sieve, we got 1 strains with strong antagonistic activity of bacteria, numbered HY32, bacteriostasis diameter achieving above of 10 ram, fermentation broth bacteriostasis activity up to 81.7%. Culture tests showed it had strong antagonistic activity to 4 plant pathogens （ Cunninghamia lanceolata, Agaricodochium camellia, Fusarium proliferatum, Pestalotio psismicrospor ） . Through indoor potted test of the HY32, the results showed that compared with the control group the HY32 has significant control effect against Chinese fir anthracnose, reaching 60.0%.