银杏内酯B对缺氧缺血性脑损伤新生大鼠脑组织Caspase-3及VEGF mRNA表达的影响

Effects of ginkgolide B on expression of Caspase-3 and VEGF mRNA in brain tissue of newborn rats with hypoxic-ischemic brain damage

目的:观察银杏内酯B(GB)对缺氧缺血性脑损伤(HIBD)新生大鼠脑组织Caspase-3及血管内皮生长因子(VEGF) mRNA表达的影响。方法:清洁级新生7d龄SD大鼠96只,随机分为假手术组、模型组、GB低剂量组及GB高剂量组。后3组采用经典Rice法制作HIBD动物模型,假手术组不行缺氧处理。模型制备4h后GB高、低剂量组分别按10、5mg/kg腹腔注射GB,余2组注射等量生理盐水,1次/d,共5d。造模后第3、7、14和28天每组分别随机选取6只动物处死,荧光定量PCR法检测脑组织Caspase-3及VEGF mRNA的表达。结果:造模后,假手术组各时间点Caspase-3和VEGF mRNA表达水平差异均无统计学意义(F=0.134,0.382,P均>0.05),而模型组、GB低和高剂量组Caspase-3和VEGF mRNA的表达均于造模后第3天达最高,此后逐渐下降,至造模后第28天接近假手术组水平(F=109.356,33.281,9.703和39.945,103.903,60.956,P<0.001)。造模后第3、7和14天,4组Caspase-3和VEGFmRNA表达水平差异均有统计学意义(F=128.865和97.912,40.058和57.121,24.184和8.621,P均<0.001);与假手术组比较,模型组Caspase-3和VEGFmRNA的表达均升高(P<0.05);与模型组比较,GB低和高剂量组Caspase-3的表达降低,VEGF mRNA的表达升高(P<0.05);GB高剂量组2指标的变化较低剂量组更明显(P<0.05)。结论:GB可下调HIBD后脑组织Caspase-3表达及上调VEGF表达,从而减轻并修复脑损伤。

Aim： To observe the effect of ginkgolide B （ GB） on mRNA expression of Caspase-3 and vascular endothe-lial growth factor （ VEGF） in brain tissue of hypoxic-ischemic brain damage （ HIBD） newborn rats. Methods： A total of 96 clean 7-day-old health SD rats were randomly divided into sham operation group,the model group,low and high dose GB treatment groups. Classic Rice method was used to establish HIBD model in the latter 3 groups. 4 h after operation,GB at dose of 5 mg / kg and 10 mg / kg was given to rats in the low and high dose GB treatment groups by intraperitoneal injection postoperation,once a day for 5 days,sham operation and model groups were given equal physiological saline. 6 rats in each group were sacrificed at the 3rd,7th,14th and 28th day after model establishment,respectively. Quantitative real-time fluo-rescent PCR was employed to detect the expressions of Caspase-3 and VEGF mRNA in brain tissue. Results： The expres-sions of Caspase-3 and VEGF mRNA had no significant differences among 4 time points in sham operation group （ F =0. 134,0. 382,P 0. 05） ,but in the model group,low and high dose GB treatment groups,the expressions of Caspase-3 and VEGF mRNA reached the peak at the 3rd day,then decreased,and reached the level of the sham operation group at the 28th day after operation（ F = 109. 356,33. 281,9. 703 and 39. 945,103. 903,60. 956,P 0. 001） . At the 3rd,7th,14th day after operation,the expressions of Caspase-3 and VEGF mRNA had significant differences among the 4 groups （ F = 128. 865 and 97. 912,40. 058 and 57. 121,24. 184 and 8. 621,P 0. 001） ; compared with sham operation group,the ex-pressions of Caspase-3 and VEGF mRNA in model group were higher,while the expression of Caspase-3 mRNA was lower and that of VEGF mRNA was higher in low and high dose GB treatment groups when compared with the model group（ P 0. 05） ,and the changes of high dose GB treatment group was more significant（ P 0. 05） . Conclusion： GB can repair brain damage after HIBD through down-regulating Caspase-3 expression and up-regulating VEGF expression.