不同蠕形螨病原学检查方法效果比较及相关因素对检出率的影响

Effects of pathogenic diagnosis of human demodex mites and related factors on detection rate

目的:探讨不同病原学检查法对人体蠕形螨的检出效果及相关因素对检出率的影响,寻找更佳的蠕形螨检查方法。方法:采用刮脂法、粘贴法及挤刮法在同一人群(共585人)的鼻部取材进行蠕形螨检查。结果:刮脂法、粘贴法及挤刮法的蠕形螨检出率分别为43.2%(253/585)、29.1%(170/585)及31.8%(186/585)。3种方法中检出率最高的是刮脂法,与其他2组比较差异均有统计学意义(χ2=74.075、45.343,P均=0.001)。3种方法不同性别患者的检出率比较,差异均无统计学意义(χ2=0.965、2.546、0.008,P均>0.05)。不同季节粘贴法的检出率比较,差异有统计学意义(χ2=3.994,P=0.046)。结论:刮脂法的检螨效果优于粘贴法和挤刮法。3种不同检查方法对男女检出率无差异。粘贴法的检出率受季节变化影响,而刮脂法和挤刮法则不受季节变化影响。

Aim： To compare the detection rate of human demodex mites with different methods and analyze the related factors on the detection rate so as to find better method of examining demodicidosis. Methods： In the same population（ n = 585） ,three kinds of different examination methods（ scraping method,transparent adhesive method,and squeezing method） were applied to detect the mite on the nose. Results： The detection rates of scraping method,transparent adhesive method, and squeezing method were separately 43. 2% （ 253 /585） ,29. 1% （ 170 /585） ,and 31. 8% （ 186 /585） . The detection rate of scraping method was significantly higher than those of the other two kinds of method（ χ 2 = 74. 075,45. 343,P = 0. 001） . The detection rate had no significant difference between different gender（ χ 2 = 0. 965,2. 546,0. 008,P 0. 05） . The de-tection rate of transparent adhesive method had significant difference among different seasons（ χ 2 = 3. 994,P = 0. 046 ） . Conclusion： The detection rate of scraping method is higher than the other two kinds of method. Different examination methods will not affect the detection rate in different genders. The detection rate of transparent adhesive method will be af-fected by different seasons while scraping method and squeezing method will not.