3种方法在梅毒实验诊断中的应用价值分析

Values of three methods in detection of syphilis

目的:探讨分析甲苯胺红不加热血清反应素试验(TRUST)、酶联免疫吸附试验(TP-ELISA)和化学发光法(TP-CMIA)在梅毒实验诊断中的应用价值。方法:收集住院患者输血前检查血清样本8000例,分别用TRUST、TP-ELISA和TP-CMIA进行梅毒抗体的检测,然后进行对比分析。结果:TRUST检出阳性397例,阳性率为4.96%;TP-ELISA检出阳性290例,阳性率为3.63%;TP-CMIA检出340例,阳性率为4.25%。TP-CMIA和TP-ELISA两者比较差异有统计学意义(P<0.05)。结论:TP-CMIA优于TRUST和TP-ELISA,具有较高的敏感性和特异性,结果观察直观、快速、省时。TP-CMIA能够助于梅毒的准确诊断,适合于常规临床筛查应用,对保障临床输血和手术安全起到积极作用。

Objective： To evaluate the clinical significance of Toluidline red unheated serum test （TRUST）, Treponema pallidum--enzyme linked immunosorbent assay （TP-ELISA） and Treponema pallidum - chemilumi- nescence immunoassay（TP-CMIA） in detection of syphilis. Method：We chose 8 000 cases of the patient in hospital. TRUST,TP-ELISA and TP-CMIA were used for each sample. And then we carried out statistical analysis. Result： The samples from 397 patient were positive for the detection with TRUST （positive rate= 4.96 %）;the samples from 290 patient were positive for the detection with ELISA （positive rate = 3.63 %）;and the samples from 340 patient were positive for CMIA （positive rate = 4. 25%）. Significant difference was found between CMIA and ELISA（P〈0.05）. Conclusion：Compared with TRUST and ELISA,CMIA is more direct,faster and more timesaving. Therefore,CMIA could be used to diagnose the syphilis accurately,and could also be combined to examine syphilis infection, especially before the blood transfusion and surgery.