大豆荚数性状相关QTL的加性、上位性及QE互作效应分析

Analysis of additive effect,epistatic and QE interaction effect for QTL of pod number traits in soybean

本研究利用Charleston×东农594得到的147个F2:14-F2:19重组自交系群体,对11个环境条件下大豆荚数性状相关QTL的加性、上位性及其与环境互作效应进行了分析。在6年11个不同遗传背景条件下的多环境联合分析中定位了11个QTL具有加性效应,其加性(A)贡献率和AE互作贡献率都是微效的。联合分析同时定位到20对QTL具有上位效应,并发现上位QTL的2种作用模式,一种是同一连锁群上2个QTL间的上位性互作,另一种是不同连锁群上2个QTL间的上位性互作。鉴定出9个具有加性效应的QTL能在多个环境条件下被检测到,17对具有上位性效应的QTL能在多个环境条件下被检测到,部分QTL的上位性效应解释的表型变异大于5%。这些在不同环境或不同遗传背景下检测到的QTL,可作为大豆荚数相关性状改良的候选标记,用于分子标记辅助选择或图位克隆。

The objective of this research is to identify QTL with additive effect and epistatic effect,and analyze the effects of additive QTL by environment（AE） interactions and epistatic QTL by environment（AAE） interactions in eleven environments over six years.The population of F2：14-F2：19 Recombination Inbred Lines（RILs）,derived from the cross between Charleston and DongNong594,was used to construct the linkage groups with Simple Sequence Repeat（SSR） markers in present study.11 additive effect QTLs（A） were identified in the conjoint analysis of 11 environments,the phenotypic variation explanations for these additive QTLs were more microefficient than that of the main effect in these additive QTLs.20 pairs of epistatic QTLs（AA） were also identified,two patterns were found between the epistatic QTLs,one was two epistatic QTLs interacted on the same linkage group,another was two epistatic QTLs located on different linkage groups interacted each other.Nine additive QTLs and 17 pairs of epistatic QTLs could be identified among multiple environments at the same time,More than 5% of the phenotypic variation could be explanted by parts of the epistatic QTLs.Therefore,the QTLs identified among different environments or different background can be used as the candidate makers for the improvement of the pod number traits in soybean in maker assisted selection（MAS） or mapping clone.