跑台运动缓解D-半乳糖AD大鼠学习记忆能力下降及其与海马ChAT、AchE的关系

Alleviation Function of Treadmill Exercise on the Decrease of Learning and Memory and Its Relationship with ChAT and AchE in Hippocampus of D-galactose AD Rats

目的:探讨8周的有氧跑台运动对D-半乳糖造模AD大鼠学习记忆能力和海马ChAT、AchE mRNA表达的影响。材料与方法:将健康雄性SD大鼠20只随机分为D-半乳糖对照组(C组,n=10)和D-半乳糖运动组(T组,n=10)。两组大鼠连续8周由腹腔注射D-半乳糖(120 mg/kg.d)制造AD动物模型。同时,对T组大鼠进行连续8周、每周5次的有氧跑台运动,第9周对两组大鼠分别进行为期6天的Morris水迷宫实验。运用实时荧光定量PCR的方法检测两组大鼠海马ChAT、AchE mRNA的表达水平。结果:8周的有氧跑台运动后T组大鼠的空间学习记忆能力提高了;同时,8周的有氧跑台运动上调了T组大鼠海马内ChAT(上调31.2%,P<0.01)的基因表达,下调了T组大鼠海马AchE(下调17.5%,P<0.05)的基因表达。结论:8周的有氧跑台运动缓解了D-半乳糖AD大鼠学习记忆能力的下降,其机制可能是由于8周的跑台运动促进了D-半乳糖模型AD大鼠海马ChAT mRNA表达的上调和AchE mRNA表达的下调,进而促进了海马Ach含量的增加。

Objective： To explore the effects of 8-week aerobic treadmill training on learning and memory and ChAT and AchE mRNA expression in hippocampus of D-galactose（D-gal） AD rats.Materials methods：20 male SD rats were randomly divided into the control group（C,n=10）,exercise group（T,n=10）.Group C and T rats were injected D-gal（120 mg/kg·d） in the abdominal for 8 weeks to make aging animal models.Group T was given aerobic treadmill,five times a week for 8 weeks.All rats participated in the Morris Water Maze（MWM） test for 6 days during the 9th week.The expression level of ChAT and AchE mRNA in hippocampus of all rats are inspected by real-time fluorescence quantitative PCR.Results： 8-week aerobic treadmill exercise may enhance the abilities of learning and memory of T group of rats in MWM and significantly up-regulate the expression of ChAT mRNA（31.2%,P0.01） and down-regulate the expression of AchE mRNA（17.5%,P0.05） in hippocampus.Conclusion： 8-week aerobic treadmill training can alleviate the decrease of learning and memory of D-gal AD rats,which may be related with the higher expression of ChAT mRNA and lower expression of AchE mRNA that may regulate the content of Ach in hippocampus of rats.