血管内皮生长因子C与血管内皮生长因子D在胃癌组织中的表达及其与淋巴管密度和淋巴结转移的关系

Expression of vascular endothelial growth factor-C and vascular endothelial growth factor-D in gastric carcinoma tissue and relationship with lymph vessel density and lymph node metastasis

目的研究血管内皮生长因子（VEGF）-C和VEGF-D在正常胃组织和胃癌组织中的表达及其与胃癌组织淋巴管密度（LVD）和淋巴结转移的关系。方法免疫组化sP法检测30例正常胃组织和75例胃癌组织中VEGF-C和VEGF-D的表达，用LYVE-1标记淋巴管，计算胃癌组织中LVD。结果VEGF-C在正常胃组织中阳性表达率为33．3％（10／30），在胃癌组织中阳性表达率为73．3％（55R5），两者比较差异有统计学意义（P〈0．01）。VEGF-D在正常胃组织中阳性表达率为30．0％（9／30），在胃癌组织中阳性表达率为66．7％（50/75），两者比较差异有统计学意义（P〈0．01）。75例胃癌患者中，有淋巴结转移40例，LVD为（8．85±1．24）个／HP，无淋巴结转移35例，LVD为（4．75±1．04）个/HP，两者比较差异有统计学意义（P=0．000）。胃癌组织中LVD随着VEGF-C和VEGF-D表达增强而逐渐升高（P〈0．01）。结论胃癌组织中LVD升高。VEGF-C和VEGF-D的高表达有利于胃癌组织淋巴管生成和淋巴结转移。

Objective To detect the expression of vascular endothelial growth factor（VEGF）-C and VEGF-D in normal gastric tissue and gastric carcinoma tissue, and further investigate the relationship between VEGF-C,VEGF-D and lymph vessel density （LVD）, lymph node metastasis. Methods The expression of VEGF-C and VEGF-D in gastric carcinoma tissues were examined by using SP immunohistochemical method in 30 normal gastric tissue and 75 gastric carcinoma tissue respectively. The lymph vessel were marked by LYVE-1 and calculated LVD. Results The rate of positive expression of VEGF-C and VEGF-D in gastric carcinoma tissue was significantly higher than that in normal gastric tissue, there was significant difference [73.3% （55/75） vs. 33.3% （10/30）, 66.7% （50/75） vs. 30.0% （9/30） ,P〈 0.01 ]. The level of LVD in gastric carcinoma tissue with lymph node metastasis （40 cases） was significantly higher than that in without lymph node metastasis （35 cases）[ （8.85 ± 1.24）/HP vs. （4.75±1.04）/HP] （P = 0.000）. The level of LVD increased when the expression level of VEGF-C and VEGF-D gradually wentup. Conclusions The level of LVD increases in gastric carcinoma tissue. VEGF-C and VEGF-D are two significant factors for LVD and lymph node metastasis.