肝再生磷酸酶-3在甲状腺乳头状癌中的表达及意义

Expression and Significance of PRL-3 in thyroid papillary carcinoma

目的:观察肝再生磷酸酶-3(phosphatase of regenerating liver 3,PRL-3)在甲状腺乳头状癌中的表达情况,并分析PRL-3的表达与各种临床病理特征的关系及意义。方法:应用real-time PCR及免疫组化SP法探索PRL-3 mRNA及蛋白在甲状腺乳头状癌原发灶、癌旁组织、淋巴结转移灶中的表达情况。结果:PRL-3 mRNA在原发灶及淋巴结转移灶中的表达均明显高于癌旁组织(P<0.05),PRL-3蛋白的阳性率在原发灶及淋巴结转移灶高于癌旁组织(P<0.05)。原发灶组织中PRL-3的阳性率在有淋巴结转移组高于无淋巴结转移组(P<0.05),肿瘤直径>2 cm的阳性率高于肿瘤直径≤2 cm(P<0.05)。结论:PRL-3与甲状腺乳头状癌的肿瘤大小及淋巴结转移密切相关。PRL-3在甲状腺乳头状癌中的高表达可以预测淋巴结转移,有望成为预测甲状腺乳头状癌淋巴结转移的分子标志物。

Objective：To investigate expression and significance of PRL-3 in thyroid papillary carcinoma and its relationship with clinicopathologic features. Methods：The expression of PRL-3 in primary thyroid papillary carcinoma,the adjacent non-tumor tissues and lymph node metastasis of the thyroid papillary cancer （LMNs） were detected by Real-time PCR and SP immunohistochemistry. Results：The transcription expression of PRL-3 mRNA was higher in primary thyroid papillary carcinoma or LMNs than in the adjacent non-tumor tissues （P 〈 0.05） .The positive expression rates of PRL-3 were also higher in primary thyroid papillary carcinoma and LMNs than in the adjacent non-tumor tissues （P 〈 0.05）. The positive rates of PRL-3 protein were higher in primary thyroid papillary carcinoma with lymph node metastasis than in primary thyroid papillary carcinoma without lymph node metastasis （P 〈 0.05）. The positive rates of PRL-3 were higher in tumor of size 〉 2 cm than in tumor of size ≤ 2 cm （P 〈 0.05）.Conclusion：The expression of PRL-3 is closely related to the tumor size and lymph node metastasis. The high expression of PRL-3 in PTC may forecast the lymph node metastasis, and it may be a molecular marker to predict lymph node metastasis in papillary thyroid carcinoma.