摘要
目的探讨原花青素对大肠癌HT-29细胞增殖及Pim-1、Bcl-2、CyclinD1基因的影响。方法取对数生长期HT-29细胞,分别加入100 mg/L、200 mg/L、400 mg/L、800 mg/L原花青素培养,采用四甲基偶氮唑蓝(MTT)法检测细胞增殖;采用荧光定量PCR检测Pim-1、Bcl-2、CyclinD1 mRNA表达。结果 24 h时,各浓度组原花青素对HT-29细胞增殖抑制率分别为(4.28±0.34)%、(10.05±0.61)%、(16.67±0.63)%、(28.16±0.91)%;48 h时,各浓度组原花青素对HT-29细胞增殖抑制率分别为(11.47±1.53)%、(17.65±1.21)%、(30.82±1.99)%、(44.48±2.18)%,对Pim-1、Bcl-2、CyclinD1mRNA表达有抑制作用(P<0.05),且作用呈浓度依赖性。结论原花青素体外可呈浓度依赖性抑制HT-29细胞增殖,其机制可能与抑制Pim-1、Bcl-2、CyclinD1表达有关。
Objective To investigate the effects of procyanidin （PA） on proliferation in human colon carcinoma cell line HT-29. Methods Human colon carcinoma cell line HT-29 of logarithm vegetal period were cultured with different density of PA（ 100,200,400,800 rag/L） for 48 hours, the suppression rate of cell pro- liferation was assayed by MTY method. The expression of Pim-1, Bcl-2, CyclinD1 was measured by fluorescent quantitative PCR respectively. Results The suppression rate of cell proliferation was （ 4. 28 ±0. 34 ） %, （ 10. 05±0. 61 ） %. （ 16. 67 ± 0. 63 ） %, （28. 16±0. 91 ） % separately at 24 hours and was （ 11.17 ±2. 36） %, （ 17.88 ±1.77 ） %. （31.65 ± 7.03 ） %, （44. 18.65 ±5.21 ） % differently at 48 hours, and the Pim-1, Bcl-2, CyclinD1 expression was reduced significantly after 48 h （P 〈 0. 05 ）. What＇s more, the effects were in a dose- dependent manner. Conclusion PA could concentration-dependently inhibit the growth of HT-29 cells,and the mechanism could possibly contribute to reduce Pim-1 ,Bcl-2,CyclinD1 expression levels.
出处
《中国实用医药》
2013年第8期4-5,共2页
China Practical Medicine
