苏州市2010—2011年志贺菌菌型分布及耐药性分析

Analysis on serotype and antimicrobial resistance of Shigella during 2010-2011 in Suzhou

目的了解苏州市志贺菌菌型分布及耐药情况,为细菌性痢疾的防治提供依据。方法用生化条鉴定菌株、血清玻片凝集确定血清型,K-B法作药敏试验。结果 103株志贺菌中鉴定出福氏志贺菌60株,其中F2a占41.67%;宋内氏志贺菌43株。药敏试验结果显示,奈啶酸耐药率最高为95.15%,其次氨苄西林耐药率为94.17%,四环素和复方新诺明耐药率均>80%。所有菌株对诺氟沙星高度敏感。80.58%的志贺菌对9种抗生素中的5种以上具有耐药性。结论 2010—2011年苏州市志贺菌优势菌型为F2a,宋内氏志贺菌检出率有升高趋势;志贺菌对萘啶酸耐药率最高,多重耐药现象日趋严重。

Objective To understand about distribution of Shigella serotypes and antimicrobial resistance, and to provide ev- idence for prevention and cure of bacillary dysentery. Methods Confirmation of Shigella was made using biochemical test strips. Serotyping was determined by visual examination of slide agglutination assays. Antibiotics susceptibility was performed by Kirby Bauer method. Results This study involved 103 isolates of Shigella species in 2010/2011 ,comprising 60 isolates of S. flexineri and 43 isolates S. sonnei. Shigella flexineri type 2a accounted for 41.67% of all shigella flexneri strains. Suscepti- bility tests indicated that 95.15% isolates were resistant to nalidixic acid and 94.17% to ampicillin. The resistance to both tet- racycline and cotrimoxazole were over 80%; 103 isolates of shigeIla species were highly sensitive to norfloxacin. The propor- tion of multi-resistant isolate which resisted to over five antimicrobial was 80.58%. Conclusion It was concluded that S. flexi- neri 2a was a dominant S. flexineri serotype in 2010/2011, while with the most resistance to nalidixic acid, Shigella presented the emergence of multidrug-resistant.