马齿苋醇提物和当归醇提物的抗氧化活性研究

Study on antioxidant activity of Portulaca oleracea alcohol extract and Angelica sinensis alcohol extract

目的研究马齿苋醇提物和当归醇提物的体外抗氧化作用,并比较两者合用时的抗氧化能力。方法在体外化学模拟条件下,采用Fenton反应法和模拟机体中黄嘌呤与黄嘌呤氧化酶反应系统分别测定马齿苋醇提物、当归醇提物及两者合用对羟自由基(OH.)和超氧阴离子自由基(O2-.)的清除能力。结果马齿苋和当归醇提物及两者合用对O2-.、和OH.均有良好的清除能力,且呈良好的量效关系;两者联合使用对O2-.、和OH.的清除能力明显优于单独使用;当归醇提物中阿魏酸含量与其对O2-.、和OH.清除能力呈正相关,r分别为0.954和0.932。结论当归醇提物的抗氧化能力强于马齿苋醇提物,并与阿魏酸含量的相关性好,初步确定当归中抗氧化相关活性成分主要为阿魏酸;当归醇提物和马齿苋醇提物合用具有协同作用。

OBJECTIVE To study the in vitro antioxidant activity of Portulaca oleracea alcohol extract and Angelica sinensis alcohol extract, and the combination of the two extracts was to provide the basis for the developnlent of more effective natural antioxidants. METHODS Under the chemical simulation conditions in vitro, Fenton reaction method,with xanthine and xanthine oxidase reaction system in the body were used to observe hydroxyl radicals and superoxide anion radical scavenging abilities. RESULTS Portulaca oleracea alcohol extract, Angelica sinensis alcohol extract and their mixture showed good abilities to scavenge hydroxyl radicals and superoxide anion radical in dose - dependant manner. The scavenging ability of the mixture was significantly stronger than that of extrac, t alone. Content of ferulic acid in Angelica sinensis alcohol extract and scavenging activity for O-2· and OH. were positively correlated, and correlation coefficients were 0.954 and 0.932, respectively. CONCLUSION The antioxidant ability of Angelica sinensis alcohol extract is stronger than that of Portulaca oleracea alcohol extract. Ferulic acid in Angelica sinensis is the main component responsible for the antioxidant ability. There is a synergistic effect between Angelica sinensis alcohol extract and Portulaca oleracea alcohol extract.