茶芪苓方剂有效部位群质量控制的研究

Quality control study on effective fractions of Chaqiling prescriptions

目的探讨茶芪苓方剂有效部位的质量控制方法。方法采用薄层扫描法测定茶芪苓方剂中黄芪甲苷的含量、紫外分光光度法测定茯苓多糖的含量、HPLC-UV法测定丹参酮ⅡA的含量。结果黄芪甲苷0.2～0.7μg与峰面积线性关系良好,平均回收率为98.37%,RSD=1.70%;茯苓多糖在3.286～13.143μg.mL-1线性良好,平均回收率为99.49%,RSD=0.88%;丹参酮ⅡA0.025～0.200μg与峰面积线性关系良好,平均回收率为98.38%,RSD=2.74%。结论所用方法准确、重复性好,可作为茶芪苓方剂有效部位群的质量控制方法。

OBJECTIVE To investigate methods for the quality control of Chaqiling prescriptions. METHODS Thin - layer chro- matography was used to determine astragaloside IV in the effective parts of Chaqiling prescriptions. The UV spectrophotometry was applied to determine the content of pachyman and HPLC was used to measure tanshinone II A. RESULTS The liner ranges of the methods of Astragaloside IV was 0.2 -0.7 μg. The average recovery was 98.37%, and RSD = 1.70%. The liner ranges of the method to determine glucose was 3.286 - 13.143 μg·mL^-1. The average recovery was 99.49% and RSD = 0.88%. The liner ranges of Tashinone Ⅱ A was 0.025 - 0.200 μg, the average recovery was 98.38% and RSD = 2.74%. CONCLUSION The established methods are simple, accurate and reproducible, which can be used for the quality control of Chaqiling prescriptions.