HPLC同时测定叶下珠中的槲皮素、木犀草素和山奈酚

Determination of quercetin,luteolin and kaeampferol in Phyllanthus urinaria by HPLC

目的采用HPLC同时测定叶下珠中的主要有效成分槲皮素、木犀草素和山奈酚。方法采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),柱温30℃,流动相为甲醇-0.4%磷酸(55∶45),流速1.0 mL.min-1,检测波长360nm,以外标法定量。结果槲皮素、木犀草素和山奈酚分别在40.12～320.96、20.8～166.4、32.4～259.2 mg.L-1线性关系良好。3者的平均回收率分别为99.6%、99.7%、99.8%,RSD分别为0.50%、0.15%、0.28%。供试品溶液在8 h内稳定。结论所用方法准确、灵敏,对进一步完善叶下珠药材的质量控制具有重要的价值。

OBJECTIVE To establish an HPLC method for simultaneously determining quercetin, luteolin and kaeampferol of Phyl- lanthus urinaria L. METHODS The sample was separated on a Agilent Eclipse XDB - C18 （250mm×4.6mm,5 μm） column at the temperature of 30℃. The mobile phase was a mixture of methanol and 0.4% phosphoric acid water solution（55：45） with a flow- rate of 1.0mL·min^-1 and the detection wavelength was 360 nm. RESULTS The calibration curves of three flavonoids were linear in the range of 40.12 - 320.96, 20.8 - 166.4 and 32.4 - 259.2 rag- L- 1, respectively. Their average recoveries were 99.6% , 99.7% and 99.8% with corresponding RSD of 0.50% , 0.15% and 0.28% , respectively. The sample solution was stable within 8 hours： CONCLUSION The method is accurate with high sensitivity and is of great value to control the quality of Phyllanthus urinaria L.