摘要
目的:建立金铁锁药材及其混淆品有效的鉴别方法,分析二者在显微特征、DNA信息特征等方面的差异,为精确鉴定金铁锁药材及其混淆品,以保障用药安全奠定基础。方法:采用中药系统鉴定,对金铁锁药材及其市售混淆品样本的显微特征及其ITS序列进行BLAST比对分析、ITS序列的特异位点差异分析,N-J系统发育树分析等,建立金铁锁及其混伪品的系统鉴定方法。结果:中药系统鉴定法的显微特征中,金铁锁正品药材中无草酸钙结晶,而其混淆品瓦草中具有大量的草酸钙簇晶分布,可作为其鉴定的重要依据之一;此外,金铁锁药材ITS序列与瓦草ITS序列差异明显,采用BLAST比对分析、N-J系统发育树分析等可将二者进一步精确鉴别。结论:采用中药系统鉴定法能够很好的对金铁锁及其混伪品进行精确的鉴别。
Objective： To develop an effective identification method for accurately discriminating Psamrnosilene tunicoides and its confused species by the combined method of microscopic identification and molecular identification, so-called systematic identifica- tion of Chinese materia medica （SICMM）. Method： P. tunicoides and its confused species were accurately discriminated by SICMM method, which was established by comprehensively use of microscopic identification and DNA identification method. The DNA identifi- cation included the following analysis： the BLAST alignment, specific bases and N-J phylogenetic tree analysis. Result： The cluster crystals were not observed in P. tunicoides, but great deals of them were found in Silene viscidula. Further more, big differences of ITS sequence were observed and analyzed between P. tunicoides and its confused specie of S. viscidula. Conclusion： The system method is a scientific and accurate method for the identification of P. tunicoides and its counterfeit species.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第8期1134-1137,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81274011)
北京市自然科学基金项目(7112076)
关键词
金铁锁
瓦草
显微特征
ITS序列
Psammosilene tunicoides
Silene viscidula
microscopic identification
ITS sequence
