补骨脂水煎液在大鼠体内组织分布的研究

Study on tissue distribution of Psoraleae Fructus decoction in rats

采用先进的技术手段阐明中药的归经理论是中医药理论研究中的重要部分。该文采用现代药物动力学的方法,考察了补骨脂中补骨脂素和异补骨脂素在大鼠体内组织分布的特性,为探讨中药的归经理论提供了研究思路和实验依据。该文一次性灌胃给予大鼠补骨脂水煎液,采用HPLC测定不同时间大鼠体内心、肝、脾、肺、肾、脑和睾丸等组织中的补骨脂素和异补骨脂素的浓度,应用DAS 2.0软件计算药动学参数。研究结果表明,补骨脂素和异补骨脂素在大鼠体内不同组织中的HPLC方法学考察指标符合生物样品测定要求,2种成分在心、肝、脾、肺、肾、脑和睾丸中均有分布,AUC0-t分别为肝>肺≈肾>心>脑>脾>睾丸、肝>肾>肺>心>脑≈脾>睾丸,肝、肾和肺中2种成分的AUC0-t与其他组织比较有显著性差异(P<0.05),2种成分在各个组织中的平均滞留时间为10 h左右,补骨脂水煎液中补骨脂素和异补骨脂素对肝、肾和肺有较强的靶向选择性。

Advanced technologies are used to clarify the meridian tropism theory of traditional Chinese medicine is an impor- tant part of theoretical studies of traditional Chinese medicine. In this article, modern pharmaeokinetic method was used to investigate tissue distribution characteristics of psoralen and isopsoralen of Psoraleae Fructus decoction in rats, in order to provide research ideas and experimental basis for the meridian tropism theory. In this study, various tissue samples such as heart, liver, spleen, lung, kid- ney, brain and spermary were collected at different times after oral administration with FP decoction, in order to determine concentra- tion of psoralen and isopsoralen by HPLC. Pharmacokinetic parameters were calculated by DAS 2. 0 software. The study results showed that HPLC indexes of psoralen and isopsoralen in various tissues of rats met the determination requirements of biological samples. Both components were distributed in all of the tissues, with AUC0-t, order of liver 〉 lung ≈ kidney 〉 heart 〉 brain ≈ spleen 〉 spermary. There was significant difference between liver, kidney, lung and other tissues （P 〈 0.05 ）. MRT0-t, of both psoralen and isopsoralen were about 10 h. Therefore, psoralen and isopsoralen showed stronger targeting selection in liver, kidney and lung.