枯草芽孢杆菌产β-1,3-1,4-葡聚糖酶的响应面优化

Optimization of fermentation conditions for β-1,3-1,4-glucanase production by the thermophilic Bacillus subtilis D-6 using the response surface methodology

【目的】采用响应面法(RSM)优化枯草芽孢杆菌5 L发酵罐产β-1,3-1,4-葡聚糖酶的发酵条件。【方法】利用Box-Behnken设计和方差分析。【结果】获得最佳发酵条件为:转速、通气量和培养基pH分别为500 r/min、1.05 vvm和5.08,发酵时间仅为22 h产β-1,3-1,4-葡聚糖酶活力达2 294.4 U/mL。【结论】实验结果表明响应面法优化5 L发酵罐发酵产β-1,3-1,4-葡聚糖酶的条件合理可行。

[Objective] [Methods] The fermentation parameters on the β-1,3-1,4-glucanase production by Bacillus subtilis D-6 in a batch fermentor （5 L） were optimized by statistical analysis using response surface methodology （RSM） based on Box-Behnken design （BBD） analysis of variance （ANOVA）. [Results] The results showed that under the optimized condi- tions, with agitation, aeration, and pH at 500 r/min, 1.05 vvm and 5.08, respectively, the high- est β-1,3-1,4-glucanase activity of 2 294.4 U/mL was obtained after 22 h of cultivation. [Con- clusion] This study proved that RSM could efficiently be applied for modeling of β-1,3-1,4-glucanase production in submerged fermentation.