辽宁省番茄细菌性斑疹病的病原鉴定

Pathogen identification of bacterial speck of tomato in Liaoning

【目的】对在辽宁部分地区采集的番茄细菌性病害病原菌进行鉴定。【方法】对病原菌进行革兰氏染色、培养性状、生理生化特性、16S rRNA基因序列测定以及hrpZpst基因特异性扩增。【结果】鉴定该病原菌为丁香假单胞杆菌番茄致病变种[Pseudomonassyringae pv.tomato(Okabe)Young,Dye&Wilkie]。【结论】利用hrpZpst基因特异性扩增可以快速鉴定病原菌。

[Objective] Identification of bacterial disease of tomato in Liaoning. [Methods] According to result of the pathogenicity, physiological and biochemical characterizations, 16S rDNA sequence and specific amplification of hrpZpst gene. [Results] The isolates were iden- tified as Pseudomonas syringae pv. tomato （Okabe）. [Conclusion] hrpZpst gene can be used to identify Pseudomonas syringae pv. tomato （Okabe）.