新型表面聚合型手性固定相的制备及应用

Preparation and application of the novel chiral stationary phase by surface-initiated polymerization

采用(S)-2-氨甲基四氢吡咯作为手性单体,以键合在3-氨丙基硅胶上的4,4'-偶氮-二(4-氰基)戊酸(ACVA)作为引发剂,通过直接引发表面聚合反应,制备了一种新型HPLC刷型手性固定相CSP-a。采用乙腈-甲醇为流动相,在柱温30℃和UV 254 nm检测条件下实现了对联萘酚衍生物、氨基酸衍生物的色谱拆分。结果表明,CSP-a对π酸和π碱类化合物都有明显拆分效果。

A novel brush-type chiral stationary phase（CSP-a） for HPLC were prepared by surfaced-initiated polymerization with（S）-2-aminomethylpyrrolidine as a chiral monomer and dichloride of 4,4′-azo-bis-4-cyanopentanoic acid bonded on the surface of silica gel as a radical initiator.The resolutions of the enantiomers of binaphthol derivatives and amino acid derivatives on CSP-a were carried out using acetonitrile/methanol as mobile phases under the detection conditions of column temperature of 30 ℃ and UV wavelength of 254 nm.The results indicate that CSP-a was effective for the resolutions of the enantiomers of π-acid and π-base derivatives.