3种超排方案生产奶牛体内性控胚胎效果比较

Comparison of Three Protocols for Producing Sexing-Embryo in Vivo for Dairy Cows

采用3种不同方案对16头经产奶牛实施超数排卵,然后用X性控冻精进行人工授精。结果显示,3种方案超排头均排卵数分别为10.75、6.00、7.00枚,头均回收胚胎数分别为8.38、4.25、7.00枚,3组均无显著差异(P>0.05);方案Ⅰ超排供体头均获可用胚7.88枚,可用枚率为95.5%,显著高于方案Ⅱ(2.20枚,55.7%)和方案Ⅲ(0.33枚,15.7%)(P<0.05)。方案Ⅱ和方案Ⅲ在头均可用胚数方面无显著差异(P>0.05),但在可用胚率方面,方案Ⅱ显著高于方案Ⅲ(P<0.05)。

A total of 16 multiparaous dairy cows were randomly divided into 3 groups and superovulated with three different protocols, and then inseminated with X-sorted sperms. The results showed that among these 3 groups,no significant difference was observed in the average number of corpus luteum （10.75 vs 6.00 vs 7.00） ,and recovered embryo/ovum per cow（8. 38 vs 4.25 vs 7.0） （P〉0.05）. The average number of recovered transferable embryo and the transferable embryo rate （number of transferable embryo/number of recovered embryo） with protocol Ⅰ were significantly higher than those of protocol Ⅱ and Ⅲ（7.88 vs 2.20 vs 0.33,95.5% vs 55.7% vs 15.7%） （P〈0.05）. There was no significant difference between protocol Ⅱ and protocol Ⅲ in the average number of transferable embryo（P〉0.05）, but the transferable embryo rate of protocol Ⅱ was higher than that of protocol m （P〈0. 05）.